宫颈上皮内瘤变组织人乳头瘤病毒感染与凋亡抑制基因表达的相关性研究  

Correlation between expression of apoptosis gene in cervical intraepithelial neoplasia and human papillomavirus infection

作　　者：胡红波[1] 许红雁[1] 黄志锋[1] 邓雪莲[1] 刘文清[2] 钟永红[1] 胡卫中[1] 邱俊勇[2] 林仲秋[3] 

机构地区：[1]广东省韶关市粤北人民医院妇产科,512026 [2]广东省韶关市粤北人民医院病理科,512026 [3]中山大学第二附属医院

出　　处：《中国综合临床》2006年第1期71-73,共3页Clinical Medicine of China

基　　金：广东省医学科研项目(A2002731)

摘　　要：目的探讨宫颈癌发生、发展过程中人乳头瘤病毒(HPV)感染与凋亡抑制基因Bcl2表达的关系。方法采用免疫组化法检测正常宫颈、慢性宫颈炎及宫颈上皮内瘤样病变Ⅰ级(CINⅠ)、CINⅡ、CINⅢ共116例组织中凋亡抑制基因Bcl2的表达;聚合酶链反应法检测HPV16、18型的存在。结果①Bcl2在正常宫颈、慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ组织中的表达呈上升趋势,CINⅡ、CINⅢ组织中Bcl2的阳性率显著高于正常宫颈、慢性宫颈炎、CINⅠ组织(均为P<0.05)。②HPV阳性组中,CINⅡ、CINⅢ组织的Bcl2的阳性率显著高于阴性率(P<0.05);CINⅡ、CINⅢ组织的Bcl2的阳性表达率与HPV感染呈正相关。结论HPV感染可能通过诱导Bcl2的过度表达,抑制细胞凋亡,促进组织增殖从而在宫颈癌发生的早期起重要作用。Objective To study the correlation between expression of apoptosis gene Bcl-2 in cervical intraepithelial neoplasia and human papillomavirus （HPV） infection. Methods Immunohistochemical SP method was used to detect the expression of BcI-2 gene in normal cervix uteri tissue,chronic cervitis,cervical intraepithelial neoplasia-Ⅰ （CIN-Ⅰ） ,CIN-Ⅱ,and CIN-Ⅲ of 116 cases. HPV16 and HPV 18 were detected with PCR. Results The expression of Bcl-2 expression was significantly increased in normal cervix uteri tissue, chronic cervitis, CIN-Ⅰ CIN-Ⅱ, and CIN-Ⅲ tissue,and the positive rate of Bcl-2 was significantly higher in CINⅡ and CIN Ⅲ than in normal cervix uteri tissue, chronic cervitis, and CIN-Ⅰ tissue （ P 〈 0.05 for each ）. In HPV positive group,the positive rates of Bcl- 2 in CINⅡ and CINⅢ tissue were significantly higher than negative rate （ P 〈 0.05 ）. The positive rate of Bcl-2 in CINⅡ and CIN Ⅲ tissue was positively correlated with HPV infection. Conclusion HPV infection plays an important role in the development of cervical carcinoma through inducing Bcl-2 overexpression,suppressing apoptosis and accelerating proliferation.

关 键 词：宫颈癌 人孔头瘤病毒 BCL-2 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]