大肠癌患者TPEF基因甲基化状态分析  

TPEF gene methylation analysis in patients with colorectal tumor

作　　者：朱斌[1] 陈尔齐[2] 楚建军 鹿亚超[1] 

机构地区：[1]苏州大学医学遗传研究室,215007 [2]苏州大学医学院 [3]无锡四院肿瘤研究所

出　　处：《江苏医药》2006年第1期7-8,共2页Jiangsu Medical Journal

基　　金：江苏省卫生厅重大项目(K2005005)

摘　　要：目的分析大肠癌患者含表皮生长因子和卵泡抑素结构域的跨膜蛋白(TPEF)基因甲基化状态,寻找新的早期诊断大肠癌分子标志物。方法常规方法提取大肠癌标本DNA,采用甲基化特异性PCR技术分析TPEF基因甲基化状态。结果32例大肠癌手术切除标本,24例检测到异常TPEF基因甲基化(75%),而10例正常组织标本无一例检测到异常甲基化。结论大肠癌患者TPEF基因甲基化为一频繁发生事件,可望成为大肠癌早期诊断的一个标志物。Objective The methylation status of transmembrane protein endothalial factor （TPEF） gene was analyzed for finding a new molecular diagnostic marker of eolorectal tumor. Methods Genomic DNA was isolated from tumor samples using a standard procedure, and TPEF methylation status was analyzed with methylation-specific PCR. Results Abnormal TPEF methylation was detected in 24 （75%） from 32 samples obtained from patients underwent operation, but no one in 10 normal samplcs. Conclusion Abnormal methylation of TPEF gene is a frequent event and may become a promising diagnostic marker for early detecting colorectal carcinoma.

关 键 词：大肠癌 TPEF基因 甲基化 肿瘤标志物 

分 类 号：R735.34[医药卫生—肿瘤] R394[医药卫生—临床医学]