DADAG诱导白血病L1210细胞凋亡  

DADAG induces apoptosis of L1210 leukemia cells

作　　者：杨锦南[1] 刘凤歧[1] 赵营[1] 催泰震[1] 

机构地区：[1]河南新乡医学院药学院,453003

出　　处：《江苏医药》2006年第1期47-49,共3页Jiangsu Medical Journal

摘　　要：目的以小鼠白血病细胞系L1210为对象,探讨半胱天冬氨酸蛋白酶(Caspases)家族成员在二乙酰二脱水卫矛醇(diacetyldianhydrogalactitol,DADAG)诱导肿瘤细胞凋亡过程中的作用。方法MTT法测定DADAG对L1210细胞的杀伤作用;透射电镜和流式细胞术检测细胞凋亡;Western blot和Caspase-3试剂盒测定Caspase-3活性。结果与对照组相比,L1210细胞经不同浓度的DADAG(12.0、17.2、24.5、35.0或50.0 mg/L)处理72 h后,其存活率,分别降至72%、63%、46%、35%和20%。DADAG 50.0 mg/L处理组的凋亡率、Caspase-3活性和PARP裂解片段较对照组明显升高。Caspase-3抑制剂可部分阻断此过程,而Caspases抑制剂可完全阻断此过程。结论Caspases家族成员在DADAG诱导L1210细胞凋亡中发挥重要的作用。Objective To investigate the possible role of eystein asparate proteases（Caspases） family members in DADAG（diacetyldianhydrogalactitol）-indueed apoptosis of mouse leukemia L1210 alls. Methods The cytotoxic effect of DADAG on L1210 cells was determined by MTT assay. DAD AG-induced apoptosis in L1210 cells was identified by flow cytometry and electron microscopy. Caspase-3 activity was measured by Western blot and ApoAlert CPP32 colorimetric assay kit. Results After incubation of 1.1210 cells with 12.0, 17.2, 24. survival rates, compared with control, progressively 5,35.0 or 50.0 mg/L DADAG for 72 h, the reduced to 72%, 63%, 46%, 35% or 20%, respectively. The apoptotie ratio, Caspase-3 activity and the amount of PARP fragments in L1210 cells were time-dependently reduced much more after treatment with DADAG 50. 0 mg/L than those in the control. Caspase-3 inhibitor partially blocked the process, while Caspases inhibitor entirely blocked the process. Conclusion Caspases family members play an important role in DADACrinduced apoptosis of leukemia L1210 cells.

关 键 词：二乙酰二脱水卫矛醇 L1210细胞 凋亡 半胱天冬氨酸蛋白酶 

分 类 号：R73-361[医药卫生—肿瘤] R979.1[医药卫生—临床医学]