PTEN基因突变与胃癌临床分期相关性的探讨  

PTEN gene mutation in gastric carcinoma

作　　者：邹明瑾[1] 高杨[1] 罗兵[2] 梁华[2] 李燕[1] 

机构地区：[1]山东大学齐鲁医院检验科,山东济南250012 [2]青岛大学医学院分子病毒学实验室,山东青岛266012

出　　处：《中国现代普通外科进展》2005年第6期352-354,共3页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:分析抑癌基因PTEN突变高发区外显子5(exon5)和外显子8(exon8)在胃癌组织中的突变频率,探讨其突变与胃癌临床分期的相关性。方法:应用聚合酶链反应-单链构像多态性分析(PCR-SSCP)方法检测胃癌组织和相应癌旁正常组织中PTEN基因的突变,对突变样本的PCR产物进行测序分析。结果:42例胃癌组织中检测到PTEN基因突变3例,突变率为7.14%(3/42);42例胃癌临床病理分期,Ⅰ、Ⅱ期突变率为5.88%(1/17),Ⅲ、Ⅳ期突变率为8.00%(2/25),二者差异无统计学意义(P〉0.05)。42例相应癌旁正常组织未检测到突变。42例胃癌进行组织学分级,低分化腺癌突变率为12.00%(3/25),中、高分化腺癌突变率为0(0/17),二者差异无统计学意义(P〉0.05)。结论:胃癌组织中存在PTEN基因突变,主要发生在低分化腺癌中,而PTEN基因突变与胃癌病理组织学分级和临床病理分期之间无明显相关性。Objective：TO study the mutation frequencies of the exon 5 and the exon 8 of PTEN （phosphatase and tensin homology deleted on chromosome ten） gene in gastric carcinoma and investigate the relationship of the gene mutation and pathological differentiation and clinical stage. Methods ：The mutation of exon 5 and exon 8 of PTEN gene was detected in 42 gastric carcinoma samples and the matched adjacent normal gastric muoosa with polymerase chain reaction-single strand conformation polymorlahism（POR-SSOP） method, The PCR products of mutant samples were analysed by DNA sequencing technique. Results ： The mutation of PTEN was shown in 3 of the 42 Bast.rio carclnoma tissues and in none of the adjacent normal tissues. The mutation rates of PTEN gene in poorly differentiated and well differentiated samcles were 12. 00% and 0, respectively （P〉0. 05）. The mutation rates of PTIEN gene in clinical stage Ⅰ and Ⅱ （5.8896） had no significant difference with that in clinicat stage Ⅲ and Ⅳ （8. 00%） （P〉0. 05）. Conclusion ：PTEN gene mutation occurs mainly in poorly differentiated gastric carcinoma tissues, and the mutation rate is not relate＇d to pathological differentiation and clinical stage.

关 键 词：胃肿瘤 基因 转变 基因 PTEN 

分 类 号：R735.2[医药卫生—肿瘤]