斑蝥灸对肺癌小鼠nm23-1基因表达的实验  

Experiment on expression of nm23-1 gene in lung cancer mice affected by cantharidin

作　　者：李学武[1] 李南玲[2] 赵立岩[1] 李延青[3] 

机构地区：[1]北京中医药大学针灸学院临床教学部,北京市100029 [2]北京中医药大学针灸学院硕士研究生班,北京市100029 [3]北京中医药大学针灸学院博士研究生班,北京市100029

出　　处：《中国临床康复》2005年第47期96-98,i0005,共4页Chinese Journal of Clinical Rehabilitation

基　　金：教育部高等学校博士学科点专项科研基金资助课题(20020026012)~~

摘　　要：目的观察斑蝥灸对肿瘤抑制率和肿瘤转移抑制基因nm23-1的m RNA表达的影响。方法实验于2004-05/08在针灸学院实验室和广安门医院分子生物实验室进行。实验动物C57BL/6J近交系小鼠50只,雌雄各半。随机分成5组,每组10只。正常对照组不做造模及治疗处理;肺癌模型组接种肺癌瘤株,不做治疗处理;单纯化疗组;单纯斑蝥灸组;斑蝥灸+化疗组。后4组接种Lewis肺癌瘤株,建立Lewis肺癌模型。用实时荧光定量反转录聚合酶链反应技术检测肺癌小鼠nm23-1基因的m RNA表达。结果各组小鼠nm23-1基因的m RNA表达量和nm23-1与β-actin基因的m RNA表达5组动物45只进行结果分析。①斑蝥灸组显著提高Lewis肺癌小鼠nm23-1基因的m RNA表达,与各自β-actin基因的m RNA表达比值的均值为5.68。②单纯化疗组和斑蝥灸配合化疗组与对照组比较,差异无显著性意义,但斑蝥灸配合化疗组明显优于单纯化疗组,其均值分别为4.35和1.8。③斑蝥灸配合化疗组对Lewis肺癌的抑制率是40.55%,优于单纯化疗组的31.09%。结论斑蝥灸配合化疗对Lewis肺癌的生长有明显的抑制作用,其作用优于单纯化疗组,说明斑蝥灸可以增强化疗药物的抗癌作用。其作用可能与促进nm23-1基因的m RNA表达有关。AIM： To observe the effects of moxibustion of cantharidin on the expression of mRNA of nm23-1 gene. METHODS： The experiment was conducted at the laboratory of Acupuncture College and biochemical laboratory of Guanganmen Hospital from May to August 2004. Experimental animals： Fifty C57BL/6J inbred line male and female （half and half） mice were selected, and assigned randomly into 5 groups with 10 rats in each group. Normal control group： The rats were not treated with therapy or building models; Lung cancer model group： The rats were inoculated with tumor of lung cancer without therapy; Pure chemiotherapy group; Pure cantharidin group; Cantharidin ＋ chemiotherapy group. The rats in the later 4 groups were inoculated with Lewis lung cancer tumor to build Lewis lung cancer model. The expression of mRNA nm23-1 gene in lung cancer mice was detected with real-time fluorescent quantitative reverse transcription polymerase chain reaction （RT-PCR） method. RESULTS： The expression of mRNA of nm23-1 gene in mice of every group and nm23-1 with expression of mRNA of β-actin gene in 45 animals of 5 groups were involved in the result analysis. （1） The expression of mRNA of nm23-1 gene in Lewis lung cancer mice enhanced significantly in the cantharidin group, and the mean value of ratio with the expression of mRNA of every β-actin was 5.68. （2） The difference had insignificant meaning as compared between pure chemiotherapy group and cantharidin ＋ chemiotherapy group with control group. But it was better in the cantharidin ＋ chemiotherapy group than that in the pure chemiotherapy group, and its mean value was 4.35 and 1.8, respectively. （3） The inhibitive rate of Lewis lung cancer in the cantharidin ＋ chemiotherapy group was 40.55%, which was better than 31.09% in the pure chemiotherapy group. CONCLUSION： The cantharidin matching with chemiotherapy has obviously inhibitive effects on the growth of Lewis lung cancer, which is better than that in the pure chemiotherapy group. It is indicat

关 键 词：斑蝥 灸法 肺肿瘤 

分 类 号：R734.2[医药卫生—肿瘤]