啤酒对大鼠脂肪代谢相关酶活性的影响(英文)  

Effects of beer on enzyme activity related to fat metabolism of rats

作　　者：顾洪雁[1] 陶如[1] 翟静[1] 孙凌云[1] 王涛[1] 柏素云[1] 

机构地区：[1]泰山医学院生物化学教研室,山东省泰安市271000

出　　处：《中国临床康复》2005年第47期159-161,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景长期过量饮用啤酒有可能会引起体内组织或血清酶活性的变化。目的观察啤酒的饮用量与大鼠脂肪合成与分解代谢相关酶活性的关系。设计随机对照动物实验。单位泰山医学院基础医学研究所。材料实验于2004-12/2005-02在泰山医学院基础医学研究所完成。选择SD大鼠60只,分为6组,每组10只。按体质量每天灌服啤酒量分为9m L/kg,18m L/kg,27mL/kg,36m L/kg及45m L/kg组;对照组大鼠食水自由,不灌服啤酒。方法各组大鼠连续喂饲1周后,麻醉处死大鼠,采取血样,留取肝脏、皮下脂肪、肠系膜脂肪组织以及腓肠肌,分别进行生化分析和酶活性的测定。主要观察指标①喂饲1周后各组大鼠体质量、血糖、胰岛素以及血脂水平。②喂饲1周后各组大鼠肝脏和脂肪组织酶活性。③喂饲1周后各组大鼠激素敏感脂肪酶和脂蛋白脂肪酶活性。结果纳入60只大鼠全部进入结果分析,无脱失。①喂饲1周后各组大鼠体质量及生化指标水平比较啤酒36m L/kg组大鼠体质量、血清游离脂肪酸、高密度脂蛋白胆固醇、肝脏三酰甘油及肝脏胆固醇含量均显著高于对照组(χ2=19.44～20.01,P<0.01)。②喂饲1周后各组大鼠肝脏和脂肪组织酶活性比较啤酒36m L/kg组大鼠肝脏、皮下脂肪组织和肠系膜组织的肝脏微粒体三酰甘油转换蛋白、磷脂酰磷酸水解酶、苹果酸酶和葡萄糖-6-磷酸脱氢酶的活性均显著高于对照组(χ2=15.02～16.00,P<0.05)。③喂饲1周后各组大鼠激素敏感脂肪酶和脂蛋白脂肪酶活性比较啤酒36m L/kg组大鼠腓肠肌激素敏感脂肪酶活性显著低于对照组(P<0.01),但皮下脂肪组织的激素敏感脂肪酶活性显著高于对照组(P<0.01)。啤酒36m L/kg组在肠系膜脂肪、皮下脂肪及腓肠肌组织中脂蛋白脂肪酶活性均显著高于其他啤酒组和对照组(χ2=19.00～20.00,P<0.01)。结论每日灌服一定量的啤酒(36m L/kg)可促进大鼠肝脏合成BACKGROUND： Long-term excessive intake of beer might lead to change of intra-corporal tissue or activity of serum enzyme. OBJECTIVE： Observation on relations between intake of beer and fat synthesis of rats and activity of enzyme correlated with catabolism in rats. DESIGN： Matched observations randomly of animal experiments. SETTING： Basic Medical Institute of Taishan Medical College, MATERIALS： The experiments were completed in Basic Medical Institute of Taishan Medical College from December 2004 to February 2005. Totally 60 SD rats were selected and categorized into 6 groups with 10 rats in each group. The rats were perfused with 9mL/kg, 18 mL/kg, 27 mL/kg, 36 mL/ kg, and 45 mL/kg beer respectively according to their fat; The rats in control group were fed with water in stead of beer. METHODS： All rats in each groups were narcotized and executed after continuous feeding 1 week, biochemical analysis and enzyme assay were made respectively after blood samples were adopted, and liver, subcuta- neous fat, mesentery fat tissue and gastrocnemius muscle were preserved. MAIN OUTCOME MEASURES： （1） The level of fat, blood glucose, insulin and blood-lipid of rats in each group after feeding 1 week .（2） The enzymatic activity of liver and fat tissue of rats in each group after feeding 1 week. （3） The activity of hormone sensitive lipase and lipoprotein lipase （LPL） of rats in each group after feeding 1 week. RESULTS： Totally 60 rats were channeled into result analysis without any loss. （1） Comparison of the levels of fat and biochemical specifications of rats in each group after feeding 1 week： The contents of fat, serum free fat- ty acid （FFA）, high-density of lipoprotein （LP） cholesterol, hepar triacylglycerol and liver cholesterol in beer 36 mL/kg group were higher than those in control groups （X^2=19.44-20.01, P 〈 0.01）. （2） Comparison of the levels of the enzymatic activity of liver and fat tissue of rats in each group after feeding 1 week： The activities of li

关 键 词：肥胖症 脂肪类 啤酒 代谢 

分 类 号：R589.2[医药卫生—内分泌]