IL-2基因修饰肿瘤细胞的形态计量学研究  

Morphometric studies of the IL2 genemodified tumor cells

作　　者：刘晓丹 郭宁[1] 李秀森[1] 涂开成[1] 曹珍山[1] 杜德林[1] 毛宁[1] 

机构地区：[1]军事医学科学院基础医学研究所

出　　处：《军事医学科学院院刊》1996年第2期117-119,共3页Bulletin of the Academy of Military Medical Sciences

摘　　要：应用图像分析系统对ＩＬ２基因转移前后的小鼠肥大细胞瘤细胞系Ｐ８１５细胞及人红白血病细胞系Ｋ５６２细胞进行了形态计量学测定及组间显著性检验。结果显示，基因修饰的Ｐ８１５及Ｋ５６２细胞的形态均发生了明显的变化，其面积、周长、最大直径、最小直径、形状因子及等效直径与其亲本细胞相比有十分显著的差异，主要表现为细胞大小不均，体积增大，形状不规则。The morphometric study of P815 mouse mastocytoma cells and human erythroleukemia cell line K562 was performed before and after IL2 gene transduction by means of image analysis system. The differences between the parameters of the transduced and nontransduced cells were evaluated. The results showed that the morphology of IL2 gene modified P815 and K562 cells was remarkably changed. The parameters measured (including the area, perimeter, maximal and minimal diameters, etc.) in IL2 gene modified P815 and K562 cells were strikingly different from those in their parent cells. The results suggested that IL2 gene modification may result in morphological change of the transduced cells.

关 键 词：白细胞介素2 基因转导 形态计量 肿瘤细胞 

分 类 号：R730.3[医药卫生—肿瘤] R73-3[医药卫生—临床医学]