木霉菌诱导甜瓜抗枯萎病相关防御反应酶系的研究  

Defensive Enzyme in Melon Induced by Trichoderma Against Wilt

作　　者：黄艳青[1] 庄敬华[1] 高增贵[1] 徐韶[2] 陈捷[3] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110161 [2]云南农业大学植物保护学院,昆明650201 [3]上海交通大学农业与生物学院,上海201101

出　　处：《沈阳农业大学学报》2005年第5期546-549,共4页Journal of Shenyang Agricultural University

基　　金：农业部948项目(201056);辽宁省十五科技攻关项目(2001208001);辽宁省教育厅科技攻关项目(2004D218);上海市科委重点项目(043919364);上海市科委振兴东北成果转化项目(043958202)

摘　　要：测定了木霉菌对甜瓜枯萎病的室内及田间防效。分析了应用木霉菌后,甜瓜根组织中部分防御酶系比活力的变化。结果表明:木霉菌对甜瓜枯萎病有较好的防治效果,室内防效和田间防效分别为72.73%和79.00%,接种木霉菌后-β1,3-葡聚糖酶活性升高,挑战接种尖孢镰孢菌,苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、多酚氧化酶(PPO)酶活性有明显的增强,表明木霉菌能明显诱导甜瓜对枯萎病的抗性反应。The control effect of Trichoderma against melon wilt was done in pot and in field. The changes of activities of the defence enzymes were analyzed in roots of melon seedings after inoculation with Trichoderma. The results showed that. Trichoderma could control melon wilt efficiently, Control effect in pot and in field was 72.73% and 79.00% respectively. Inoculation with Trichoderma followed by Fusarium oxysporum was able to increase the activities of phenylalanine ammonia-lysae （PAL）, peroxidase（POD）, prophenyloxidase（PPO）. The β-1,3-glucanase was significantly greater than others. So Trichoderma could induce the resistant reaction of melon to Fusarium oxysporum.

关 键 词：木霉 甜瓜枯萎病 诱导抗性 苯丙氨酸解氨酶 过氧化物酶 多酚氧化酶 Β-1 3-葡聚糖酶 

分 类 号：S482.292[农业科学—农药学] S436.5[农业科学—植物保护]