保肝解毒颗粒对四环素所致急性肝损伤小鼠IL-18及细胞凋亡的影响  

Effect of Baogan Jiedu Granule on IL-18 and Cell Apoptosis in Model Mice with Acute Liver Injury Induced by Tetracycline

作　　者：禄保平[1] 杨晓娜[1] 许家艳[1] 

机构地区：[1]河南中医学院肝病研究所,河南郑州450008

出　　处：《河南中医学院学报》2006年第1期26-28,共3页Journal of Henan University of Chinese Medicine

基　　金：河南省科技攻关项目(编号:0324410026)

摘　　要：目的:探讨药物性肝损伤的发生机理及保肝解毒颗粒的作用机制。方法:60只小鼠随机分为模型组、保肝解毒颗粒大、中、小剂量组、甘利欣对照组和空白对照组,分别以相应剂量药物灌胃5天,然后以四环素灌胃造模,检测小鼠血清ALT、AST和IL-18水平及肝细胞凋亡情况。结果:模型组小鼠血清IL-18水平明显升高,肝细胞凋亡程度严重;保肝解毒颗粒可有效降低血清ALT、AST和IL-18水平,并使肝细胞凋亡程度明显减轻。结论:四环素所致的急性肝损伤与IL-18和肝细胞凋亡密切相关;保肝解毒颗粒可通过降低血清IL-18水平、抑制肝细胞凋亡等起到防治肝脏损害的作用。Objective： To explore the mechanisms of drug-induced liver injury and the acting mechanisms of Baogan Jiedu Granule. Method：60 mice were randomly divided into Tetracycline model group, big-dosage, medium- dosage and small-dosage group of Baogan Jiedu Granule, Ganlixin group and control group. Each was respectively perfused with corresponding drug for 5 days and Tetracycline was perfused once to make model, The levels of ALT,AST and IL- 18 in the serum were respectively measured and liver cell apoptosis was observed. Result： IL- 18 in the serum of model group rised obviously and the mice appeared to be severe apoptosis of the liver cells. Baogan Jiedu Granule could reduce the level of ALT,AST and IL- 18 obviously and showed great effect on restraining cell apoptosis. Conclusion;Acute liver injury induced by Tetracycline has close relation to IL- 18 and cell apoptosis. The mechanism of Baogan Jiedu Granule on preventing and curing acute liver injury is mainly relative to reducing the level of IL- 18 and inhibiting apoptosis of the liver cells.

关 键 词：保肝解毒颗粒 药物性肝损伤 四环素 IL-18 细胞凋亡 

分 类 号：R575.5[医药卫生—消化系统]