牛磺酸对染石英肺巨噬细胞DNA损伤的保护作用  

Protective Effects of Taurine on DNA Damage Induced by Quartz

作　　者：金玉兰[1] 阎志[2] 范雪云[1] 姚三巧[1] 白玉萍[1] 

机构地区：[1]华北煤炭医学院预防医学系,河北唐山063000 [2]天津市第四中心医院,天津300140

出　　处：《工业卫生与职业病》2006年第1期36-38,共3页Industrial Health and Occupational Diseases

摘　　要：目的探讨牛磺酸对石英致大鼠肺巨噬细胞DNA损伤的保护作用。方法将体外培养的肺巨噬细胞分成生理盐水组、石英组、牛磺酸＋石英组。用单细胞凝胶电泳技术检测各组细胞彗星出现率和彗星尾长度。结果5个浓度牛磺酸（5，10，20，30，40mmol／L）＋石英组的彗星出现率和彗星尾长度与石英组相比，差异均有显著性，且存在明显的剂量一效应关系。随着牛磺酸浓度的增加，彗星出现率降低，彗星尾长变短。结论牛磺酸在5～40mmol／L浓度范围内对石英引起的肺巨噬细胞DNA损伤有一定保护作用。Objective To study protective effects of taurine on DNA damage induced by quartz in alveolar macrophages （AM） of rats. Methods The AMs were divided into seven groups： namely the control group, quartz group, and five different doses of taurine＋quartz groups. Single cell gel elcctrophoresis was adopted to analyze the comet rate and the length of comet tail of AM. Results The comet rates of all taurine ＋quartz groups were lower than those of quartz group （P〈0.01）, and the lengths of comet tail were shorter as well （P〈0.01）o There was also significant dose-response relationship between the five different doses groups. Conclusions Taurine has protective effects on DNA damage induced by quartz in the AM of rats at a certain dosage range.

关 键 词：牛磺酸 石英 单细胞凝胶电泳技术 DNA损伤 

分 类 号：R135.2[医药卫生—劳动卫生]