氦-氖激光对体外培养神经元生长发育与存活的影响  

Effects of laser irradiation on the development and survival of cultured neurons in vitro

作　　者：吴爱群[1] 张顺清[2] 张伟宏[2] 阎志勇[2] 李宛青[3] 章萍[4] 

机构地区：[1]第二军医大学解剖学教研室,上海200433 [2]郑州大学医学院解剖学教研室 [3]郑州师范高等专科学校生命科学系 [4]郑州大学医学院物理教研室

出　　处：《中华物理医学与康复杂志》2006年第1期19-22,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：河南省科技攻关项目(No.02110438);河南省教育厅科技攻关项目(No.023100015)

摘　　要：目的研究氦-氖激光照射对培养神经元生长发育、存活及衰老的影响。方法采用氦-氖激光照射无血清培养基培养的神经元,观测其生长发育、存活、超氧化物歧化酶(SOD)、丙二醛(MDA)等的变化。结果氦-氖激光照射可促进神经元生长及其网络形成、存活时间延长、增加SOD含量和减少MDA生成。结论氦-氖激光照射可促进神经元发育、延缓神经元衰老及增强神经元的抗氧化能力。Objective To explore the effeects of He-Ne laser irradiation（HNLI） on the growth and development, survival of euhurett neurons. Methods The neurons euhured with serum-free medium were treated hy NHLI, and then the changes of growth and survival, SOD, and MDA were observed and measured after irradiation. Resuits HNLI promoted the growth and network tormation of neurons, prolonged survival period, increased the content of SOD, and reduced MDA. Conclusion HNLI could promote the development of neuron, delay the aging of neurons and increase the ability of anti-oxidation.

关 键 词：氦-氖激光 神经元培养 神经元发育 超氰化物歧化酶 丙二醛 

分 类 号：R741[医药卫生—神经病学与精神病学]