基因重组大肠杆菌生产类人胶原蛋白补氮策略优化研究  

Study on Nitrogen-feeding of Recombinant Escherichia coli for Producing Human-like Collagen

作　　者：张兮[1] 范代娣[2] 花秀夫[2] 骆艳娥[2] 马晓轩[2] 

机构地区：[1]西北大学生命科学学院,陕西西安710069 [2]西北大学化工学院,陕西西安710069

出　　处：《化学与生物工程》2006年第1期33-36,共4页Chemistry & Bioengineering

基　　金：国家科技攻关重大专项基金资助项目(2003DA901A32);国家自然科学基金资助项目(20476085)

摘　　要：优化重组大肠杆菌高密度发酵的调控工艺。利用重组大肠杆菌高密度发酵生产类人胶原蛋白,考察了连续流加和分阶段式两种不同补氮策略对细胞生长和类人胶原蛋白产量的影响。不同补氮方式显著影响类人胶原蛋白的产量。分阶段式补氮方式优于连续流加补氮方式,有利于细胞生长和类人胶原蛋白的表达,最终细胞浓度为76.01 g.L-1(DCW),类人胶原蛋白浓度为16.75 g.L-1,蛋白表达量为22.1%。Process control of recombinant Escherichia coli in fed batch culture should be optimized. Recombinant E. coli BL 21 was cultivated in high cell density to produce human-like collagen. Effect of two different nitrogen feeding modes on the cell yield and human-like collagen production were investigated. The studies showed that different nitrogen feeding modes affected human-like collagen production greatly. The cell density and concentration of human-like collagen controlled by grading nitrogen-feeding were higher than that controlled by continuous nitrogen-feeding,the concentration of cell density and human-like collagen production, ex- pressioncould reach 76.01 g·L^-1 （Dry Cell Weight）, 16.75 g· L^-1 and 22.1% respectively.

关 键 词：重组大肠杆菌 高密度发酵 类人胶原蛋白 分批补料培养 补氮 

分 类 号：Q939[生物学—微生物学]