T-DNA(Ds)插入产生的水稻卷叶突变的遗传分析  

Genetic Analysis of a Rolled Leaf Mutation by T-DNA(Ds) Insertion in Oryza sativa

作　　者：陈兆贵[1] 王江[2] 张泽民[1] 刘芳[1] 朱海涛[1] 宛新杉[2] 张景六[2] 张桂权[1] 

机构地区：[1]华南农业大学农学院广东省植物分子育种重点实验室,广东广州510642 [2]中国科学院上海生命科学研究院植物生理生态研究所植物分子遗传国家重点实验室,上海230002

出　　处：《华南农业大学学报》2006年第1期1-4,共4页Journal of South China Agricultural University

基　　金：国家重点基础研究规划(G19990116);广东省自然科学基金(04020564)

摘　　要：在筛选和鉴定水稻T-DNA(D s)插入纯合体的过程中,观察到1个叶片卷曲的突变体.对该突变体进行遗传分析表明,分离群体出现叶片卷曲和叶片正常2种植株类型,其分离比率为3∶1,符合1对基因的显性遗传.Basta抗性检测及PCR分子检测证实,该突变体是由单一T-DNA(D s)插入所引起的,突变性状与T-DNA(D s)共分离.该突变材料可用于插入座位的基因克隆.A roiled leaf mutant, Oryza sativa L. subsp, japonica IL39, caused by T-DNA （Ds） insertion was identified. Genetic analysis of the mutant showed that the two types of phenotype, rolled leaf and normal leaf in the segregating populations derived from the T-DNA （Ds） heterozygotes, fit the ratio of 3： 1. Test for Basta resistance showed that the rolled leaf plants were all resistant while the normal leaf plants were susceptible. The ratio of resistant and susceptible plants is 3：1 , which indicate that the rolled leaf mutant is co-segregated with Basta resistance. The rolled leaf mutant caused by T-DNA （Ds） insertion was further confirmed by T-DNA detection using PCR method. This rolled leaf mutant is useful for isolation of the tagged gene in rice,

关 键 词：水稻 卷叶突变体 T-DNA插入 遗传分析 

分 类 号：Q74[生物学—分子生物学]