Chemical Modification and Fluorescence Spectrum of Tryptophan Residues in Pullulanase  

作　　者：TENG Li-rong FAN Hao ZHANG Yuan-yuan YU Qi HUANG Yue-feng LIU Lan-ying 

机构地区：[1]College of Life Science, Jilin University, Changchun 130023, P. R. China

出　　处：《Chemical Research in Chinese Universities》2006年第1期61-64,共4页高等学校化学研究（英文版）

摘　　要：Tryptophan（Trp） residues in a pullulanase were modified by N-bromosuccinimide（NBS）. The results of the Spande method indicate that there are 18 Trp residues in the pullulanase and nine of them are located on the surface af the enzyme. Three of these Trp residues are nonessential residues which show the fastest reaction rate according to the Zou＇s plot. Two of the seven relative faster reacting residues are essential for the activity of the enzyme. The other eight are the slowest in the reaction rate or non-reactive residues for the reaction. The fluorescence and circular dichroism（CD） spectra of the pullulanase have been changed after the reaction with NBS. Potassium iodide（KI） and acrylamide also have remarkable influences on the fluorescence spectra of the pullulanase.Tryptophan（Trp） residues in a pullulanase were modified by N-bromosuccinimide（NBS）. The results of the Spande method indicate that there are 18 Trp residues in the pullulanase and nine of them are located on the surface af the enzyme. Three of these Trp residues are nonessential residues which show the fastest reaction rate according to the Zou＇s plot. Two of the seven relative faster reacting residues are essential for the activity of the enzyme. The other eight are the slowest in the reaction rate or non-reactive residues for the reaction. The fluorescence and circular dichroism（CD） spectra of the pullulanase have been changed after the reaction with NBS. Potassium iodide（KI） and acrylamide also have remarkable influences on the fluorescence spectra of the pullulanase.

关 键 词：PULLULANASE Tryptophan （Trp） Chemical modification Fluorescence spectrum 

分 类 号：Q556.2[生物学—生物化学] Q503