小儿退热贴祛邪作用的实验研究  

Experimental Study on Eliminating Pathogenic Effects of Infantile Umbilical Paste

作　　者：张国斌[1] 

机构地区：[1]湖北中医学院,湖北武汉430061

出　　处：《时珍国医国药》2006年第1期41-44,共4页Lishizhen Medicine and Materia Medica Research

摘　　要：目的 观察小儿退热贴的祛邪作用，为临床治疗小儿外感发热提供实验依据。方法 采用给药清除流感A型病毒感染小鼠体内病毒的方法，观察小儿退热贴对流感A型病毒感染小鼠的存活率、平均存活时间、肺指数及血凝滴度的影响；体外抗流感病毒试验采用MTT法检测病毒抑制率；体外抑菌实验（MIC测定法）观察小儿退热贴对肺克杆菌、绿脓杆菌、金黄色葡萄球菌、乙型链球菌、肺炎球菌的抑制作用；采用给药治疗葡萄球菌感染小鼠的方法，观察小儿退热贴对葡萄球菌感染小鼠的死亡率的影响。结果 小儿退热贴1．04g·kg^-1·d^-1可提高流感病毒A型感染小鼠的存活率、延长其存活时间，肺指数和血凝滴度与病毒对照组相比较具有显著性差异；小儿退热贴在浓度5～1280μg／ml的范围内，随浓度的增加，对流感病毒的抑制率也增加，并表现出量效关系；小儿退热贴含生药量0．83g/ml对肺克杆菌有较强的抑制作用、含生药量0．11g／ml对绿脓杆菌有较强的抑制作用、含生药量0．06g/ml对金黄色葡萄球菌有较强的抑制作用、对乙型链球菌和肺炎球菌没有抑制作用；小儿退热贴可降低金黄色葡萄球菌感染小鼠的死亡率，与模型对照组比较有显著性差异（P〈0．05）。结论 小儿退热贴具有体内外抗病毒、抑菌的作用。Objective To provide an experimental evident for clinical treating infantile fever, we observed the eliminating pathogenic effects of infantile umbilical paste. Methods We applied the method of giving the medicine to mice infected by influenza vires A to detect some indexes, including survival rate, mean survival time, lung index, hemagglutination titer, to evaluate the effects of eliminating virus in vivo; We applied MTT method to detect protect rate of virus in vitro; In another experiment, we evaluated the medicine inhibition to Klebsiella Pneumoniae bactedostatic, P. aeruginosa, acpococcus, beta - streptococcus, preumococcus in vitro. We gave infantile umbilical paste to mice infected by acpococcus to evaluate the medicine effects on mice mortality. Results The group of infantile umbilical paste （ 1.04 g/kg） can increase survival rate of mice infected by influenza virus prolong survial time, and its lung index and hemagglutinati on tier have a signincant difference compared with the group only treated with virus;Within the range of 5～1280μg/ml,when the medicine concentration increased,protect rate on group of infantile umbilical paste （0.83 g/ml） has stronger inhibiting effects on Klebsiella Pheumoniae,0.11 g/ml has stronger inhibiting effects on P. aemginoSa ,0.06 g/m1 has stronger inhibiting effects on acrococcus, and it has little effects on beta - streptococcus and preumococus;The medicine can bring down monality of mice infected by acrococcus. It has significant difference compared with the control group without medicine （P 〈 0. 01 ）. Conclusion Infantile umbilical paste has anti - virus and inhibiting bacteria effects in vitro and in vivo.

关 键 词：小儿退热贴 抗病毒 抑菌 

分 类 号：R285.5[医药卫生—中药学]