内切β-葡聚糖苷酶的分离纯化及酶学性质  

Purification and Properties of β-glucosidase From Therchoderma Viride WS-71

作　　者：吴石金[1] 汪劼[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,杭州310032

出　　处：《科技通报》2006年第1期51-55,共5页Bulletin of Science and Technology

基　　金：国家自然科学基金资助项目(39460060)

摘　　要：通过分子筛层析和离子交换层析等手段,分离纯化了绿色木霉WS-71纤维素酶系中的内切型β-1,4-葡聚糖苷酶组分。通过SDS-PAGE电泳测得分子量为41.8kD,该酶组分的最佳水解温度是50℃,最适pH为4.6。酶在温度40～70℃和在pH4.0￣5.0的区间稳定。Fe3+,K+,Ca2+,Mg2+,Mn2+对酶解有促进作用,Hg2+,Cu2+,Zn2+和EDTA对酶解有不同程度的抑制作用。作用于羧甲基纤维素钠的Km值为16.78mmol/L,Vmax为5.612×10-4mol/min,Kcat为6.97S-1。A β-glucosidase component from Therchoderma viride WS-71 was separated and purified by exclution chromatography and ion-exchange chromatography. By SDS-PAGE, the molecular weight of the enzyme was determined as 41.8 kD. The enzyme showed optimal activity at 50℃ and pH 4.6. It was stable in 40-70 ℃ and pH 4.0-5.0. The result showed that the Km, Vmax, Kcat was 16.78 mmol/L, 5.612×10^-4 mol/min and 6.97S^-1 when CM-cellulose was as substrate. Fe^3＋, K^＋, Ca^2＋, Mg^2＋ and Mn^2＋ can activate the enzymatic activity and Hg^2＋, Cu^2＋, Zn^2＋ and EDTA inhibitate the enzymatic activity.

关 键 词：生物化工 绿色木霉 内切型β-1 4-葡聚糖苷酶 分离纯化 酶学性质 

分 类 号：Q936[生物学—微生物学]