饥饿对异育银鲫主要器官组织游离氨基酸含量和生化组成的影响  

The changs of free amino acid in all organs and tissues of carssius aurtus during starvation

作　　者：伍代勇[1] 叶元土[1] 丁小峰[1] 蒋蓉[1] 

机构地区：[1]苏州大学生命科学学院

出　　处：《饲料工业》2006年第2期30-33,共4页Feed Industry

摘　　要：异育银鲫在饥饿60d的过程中,设置11个时间点取样,采用茚三酮方法测定异育银鲫主要器官组织游离氨基酸总量,同时测定粗脂肪、粗蛋白、水分含量,探讨在饥饿过程中各器官组织生化组成和游离氨基酸库中游离氨基酸含量的变化规律。结果发现:①饥饿过程中,鲫鱼各器官组织粗脂肪和水分含量都有所降低,不同器官组织下降的趋势不一致;而全鱼、肌肉和肝胰脏粗蛋白含量基本保持不变。②饥饿0～60d,各器官组织游离氨基酸含量变化不一致。以饥饿60d和0d值比较,降低比例依次为肠道71.09%、肝胰脏70.95%、肌肉58.14%、肾脏50.02%、心脏38.87%,降低比例较大;而脾脏、大脑、性腺含量则变化不大。③整个饥饿过程,各器官组织游离氨基酸含量并不是一直下降的,部分器官组织出现上升的过程。游离氨基酸含量呈上升趋势的时间:肌肉和肠道出现在饥饿5～10d;全鱼、肾脏出现在5～20d;而肝脏、心脏在1～3d。The Carssius Aurtus have been starved for 60 days and exampled in 0 （4h after last feeding）, 1th, 3th, 5th, 10th, 15th, 20th, 30th, 40th, 50th, 60th day respectively. The content of free amino acid （FAA） in the whole body, muscle, brain, gut, liver, spleen, kidney, heart, spermary, ovary was measured with the method of ninhydrin and at the same time the lipid, protein and water content were measured during starvation. The following conclusion was obtained：（1）The lipid and water content of all the organs and tissues decreased. There was different decreasing tendency in different organs and tissues. The protein content in the whole body, muscle and liver did not change significantly during the starvation.（2） The FAA content of different organs and tissues changed differently, which in the gut, liver, muscle, kidney, heart deceased 71.09%, 70.95%, 58.14%, 50.02%, 38.87%, respectively, comparing the value in 60th day with that in 0 day. The FAA content of spleen, ovary, brain did not changed significantly.（3） The content of FAA in some organs and tissues have not been decreasing in the whole process of starvation , while some organs and tissues which showed the increasing tendency were the muscle and gut in the 5-10th day, the whole body and kidney in the 5-20th day, and the liver and heart in the 1- 3th day.

关 键 词：鲫鱼 饥饿 游离氨基酸 

分 类 号：S965.117[农业科学—水产养殖]