大肠癌增殖细胞核抗原与核仁组成区相关性的研究  

Relationship between nucleolar organizer region and proliferating cell nuclear antigen in carcinoma of large intestine

作　　者：张纪华[1] 马述仕 李全民[1] 王美娥[1] 

机构地区：[1]北京武警总医院病理科

出　　处：《武警医学》1996年第2期68-70,共3页Medical Journal of the Chinese People's Armed Police Force

摘　　要：应用免疫组织化学方法和胶质银染色技术同时对２０例大肠腺癌，２０例腺瘤组织中增殖细胞核抗原（ＰＣＮＡ）的表达以及核仁组成区嗜银蛋白（ＡｇＮＯＲ）颗粒计数进行了检测。结果显示：大肠癌及腺瘤中的ＰＣＮＡ阳性率分别为７５％和３５％（Ｐ＜０．０５），大肠癌细胞核内的ＡｇＮＯＲ颗粒均值（６．７１±０．５９）也明显高于腺瘤的颗粒均值（３．２５±０．２３）（Ｐ＜０．０１）。说明ＰＣＮＡ的增殖程度与ＡｇＮＯＲ计数有密切的相关性，同时反映了细胞的增殖活性。因此二者联合检测，可用作早期诊断大肠癌有价值的参考指标。The expression of proliferating cell nuclear antigen (PCNA) was detected by immunohistochemical method and the number of argyrophilic nucleoalar organizer region(AgNOR) was counted by silver colloid technique in 20 patients with carcinoma of large intestine and 20 patients with adenoma of large intestine. The results showed that PCNA positive rates were 75% and 35% in carcinoma and adenoma of large intestine, respectively.The granular average value of AgNOR in carcinoma (6.71±0.59) was higher than that in adenoma(3.25± 0.231). It is indicated that PCNA proliferating rate has closed relation with the AgNOR count. Thus, combined use of both methods can be a valuable index for early diagnosis of carcinoma of large intestine.

关 键 词：大肠肿瘤 腺癌 腺瘤 PCNA AGNOR 

分 类 号：R735.34[医药卫生—肿瘤]