杜氏利什曼原虫SSU　rRNA逆转录多聚酶链反应的建立  

ESTABLISHMENT OF SSU rRNA REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION OF LEISHMANIA DONOVANI

作　　者：陈建平[1] 胡孝素[1] 杨文天[1] 

机构地区：[1]华西医科大学寄生虫学研究室,北京昌平流字五号基因工程室

出　　处：《实用寄生虫病杂志》1996年第1期1-3,共3页Journal of Practical Parasitic Diseases

基　　金：纽约中华医学基金;国家教委博士点专项基金

摘　　要：我们采用引物Ｒ２２２和Ｒ３３３建立杜氏利什曼原虫ＳＳＵｒＲＮＡ逆转录多聚酶链反应（ＲＴ－ＰＣＲ）。采用ＳｉＯ２－高盐吸附法同时抽提杜氏利什曼原虫ＲＮＡ和ＤＮＡ，分别用ＰＣＲ和ＲＴ－ＰＣＲ进行扩增，结果显示以ＲＮＡ为检测对象的ＲＴ－ＰＣＲ肉眼可见区带的检测水平较ＬＪＤＮＡ为检测对象的ＰＣＲ扩增高１００倍，可见ＲＴ－ＰＣＲ具有敏感性高、特异性强的特点。We amplified SSU rRNA of Leishmania donovani with primer R222 and R333 by reverse transcription polymerase chain reaction. PCR and RT-PCR were conducted with primer R222 and R333 to amplify L. d. SSU rRNA and SSU rDNA extracted by the method of SiO2-high concentration salt solution (8mol/L GuHCl) adsorption. It demonstrated that the detection sensitivity of RT-PCR to amplify SSU rRNA could be 100 times higher than that of PCR to amplify SSU rDNA at the same time.

关 键 词：Leiskman原虫 杜氏利什曼原虫 SSUrRNA RT-PCR 

分 类 号：R382.22[医药卫生—医学寄生虫学]