急非淋M_2型白血病单克隆抗体与高三尖杉酯碱偶合物的制备及体外对白血病细胞的杀伤作用  

Preparation of McAb 1D_1-HHT Conjugate and Its Killing Effect on Leukemia Cells in Vitro

作　　者：潘荆华[1] 王丽叶[1] 王诚[1] 李秀芝[1] 张权一[1] 

机构地区：[1]卫生部长春生物制品研究所,长春130062

出　　处：《中国生物制品学杂志》1996年第2期53-57,共5页Chinese Journal of Biologicals

摘　　要：用羟基磷灰石、DEAE－Sephacel和SephacrylS－300纯化了抗急非淋M2型白血病MCAb1D1，纯度为99．9％。用直接交联方法将McAb1D1与HHT仍联，克分子比1：40。该偶合物保持了抗体和药物活性，对急非淋M2型白血病靶细胞有选择性杀伤作用，而对急非淋M1型白血病细胞和Hela细胞无明显杀伤作用。The McAb 1D1 to acute non-lymphatic leukemia type M2 was purified by hy droxyapatite, DEAE-Sephacel and Sephacryl S-300 column chromatographys. The purified McAb 1D1 with a purity of 99. 9% was conjugated with homoharingtonine (HHT) by direct cross linking. The molar ratio of McAb 1D1 and HHT was 1: 40. The McAb 1D1-HHT still retains antibody and drug activities. It showed selective killing effect on acute non-lymphatic leukemia cells type M, but no significant killing effect on M1 type cells and Hela cells.

关 键 词：单克隆抗体 白血病 高三尖杉脂碱 免疫偶合物 

分 类 号：R733.710.3[医药卫生—肿瘤] R392-33[医药卫生—临床医学]