SD大鼠及beagle犬肾组织形态参数研究  

Studies on Renal Histological Parameters in SD Rats and Beagles

作　　者：徐勤[1] 沈逸萍[1] 苏俊芳[1] 罗振亮[1] 许安丽[1] 

机构地区：[1]广州中医药大学,广州510405

出　　处：《广州中医药大学学报》2006年第1期75-77,共3页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省教育厅科研基金(编号:Z022014)

摘　　要：了解SD大鼠及beagle犬肾组织学参数,为肾毒理病理诊断提供组织学判断依据。【方法】以40只成年SD大鼠及20只成年beagle犬为研究对象,雌雄各半,大鼠在12周龄处死,beagle犬在12月龄时处死。以体积分数为10％中性福尔马林固定肾脏,石蜡包埋,5μm切片,苏木素-伊红(HE)染色。以Spot Advanced图像分析软件测量犬和大鼠皮质及近髓肾单位、肾小球长径、短径及平均直径,同时比较种属间及不同性别动物之间的区别。【结果】beagle犬皮质肾单位肾小球长径大约为96μm,短径大约为76μm;近髓肾单位肾小球长径约为120μm,短径约为94μm,雌雄犬无显著性差异,但近髓肾单位长径、短径均大于皮质肾单位长径及短径。SD大鼠皮质肾单位肾小球长径约为87μm,短径约为64μm,近髓肾单位肾小球长径约为88μm,短径约为64μm,雌雄大鼠间无显著性差异,皮质及近髓肾单位肾小球长、短径亦无显著性差异。beagle犬组的皮质和近髓肾单位的长、短径均大于SD大鼠组(P<0．05或P<0．01)。【结论】beagle犬近髓肾单位大于皮质肾单位,而SD大鼠近髓肾单位及皮质肾单位大小无显著性差异;beagle犬肾小球较SD大鼠肾小球稍大,性别对beagle 犬及SD大鼠肾小球大小无影响。[ Objective] To investigate the renal histological parameters in SD rats and beagles and to supply evidence for renal toxicological and pathological diagnosis. [ Methods ] Forty SD adult rots and 20 adult beagles （half males and half females）, were respectively executed at 12 weeks and 12 months old. Then, kidney tissue was fixed in 10% neutral tbrmalin, embedded in paraffin, made into slices at 5 μm and stained by HE. The larger diameter and shorter diameter of glomerulus in the renal cortex and outer stripe of rats and software. The differences of the above parameters appearing beagles were determined by Spot Advanced image analysis in different species and genders were compared. [ Results] The larger diameter was about 96 μm, and the shorter diameter 76 μm in the renal cortex of the beagles, and the larger was about 120 μm and the shorter 94 μm in the outer stripe of the beagles. The differences of the larger and shorter diameters were insignificant between the male and female beagles, but the larger anti shorter diameters in the outer stripe were longer than those in the cortex. The larger diameter and shorter diameter were about 87 μm and 64 ban respectively in the renal cortex of SD rats, and the larger was about 88 μm and the shorter 64 μm in the outer stripe of SD rats. The differences of the larger and shorter diameters were insignificant between the male and female beagles, as well as in the outer stripe and the cortex. [ Conclusion] The nephron in the outer stripe is larger than that in the cortex of beagles but no difference of the nephron exists in SD rats. The nephron in beagles is larger than that in SD rats, and there is no difference in the nephron between male and female beagles and SD rats.

关 键 词：肾小球/病理学 毒理学 犬 大鼠 

分 类 号：R361.2[医药卫生—病理学]