髓系白血病细胞系HLA-Ⅱ基因型的探讨  

Study on HLA-Ⅱgenotype of myelogenous leukemia cell lines

作　　者：谢桂岚[1] 李志祥[1] 

机构地区：[1]首都医科大学附属北京友谊医院血液科,北京100050

出　　处：《中国现代医学杂志》2006年第1期18-20,共3页China Journal of Modern Medicine

摘　　要：目的检测髓系白血病细胞系HLA-Ⅱ基因型。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术对4种髓系白血病细胞系(U937、K562、HEL、MEG)的HLA-Ⅱ基因型进行DNA分型。结果该4种白血病细胞系的HLA-Ⅱ基因型分别是,U937细胞:DRB1*0301/*0301,DQB1*0201/*0201,DPB1*0402/*0402;K562细胞:DRB1*0301/*0301,DQB1*0201/*0201,DPB1*0402/*0402;HEL细胞:DRB1*1302/*1302,DQB1*0201/*0201,DPB1*1401/*1401;MEG细胞:DRB1*1406/*0410,DQB1*0401/*0401,DPB1*0501/*0501。结论该4种细胞系虽然来源于不同个体,但均属于髓系肿瘤细胞,国际FAB分类均属于急性髓系白血病细胞。然而,分子免疫遗传学类型有所差异。通过本研究提高了髓系白血病细胞系HLA-Ⅱ基因型认识,对今后髓系白血病分子免疫遗传学的研究,提供了初步资料。[Objective] To investigate the expression of HLA- Ⅱ genotype in myelogenous leukemia cell lines in order to study the relationship between HLA- Ⅱ genotype and acute myelogenous leukemia. [Methods] HLA- Ⅱ genotype of myelogenous leukemia cells lines was investigated by polymerase chain reaction - restriction fragment length polymorphism （PCR-RFLP）method. [Results] The HLA-Ⅱ genotype of K562 cells lines was as follow： DRB1 ＊ 0301/＊ 0301, DQB1 ＊ 0201/＊ 0201 ,DPB1 ＊ 0402/＊ 0402. The HLA- Ⅱ genotype of U937 cells lines was as follow： DRB1 ＊ 0301/＊ 0301, DQB1 ＊ 0201/＊ 0201 ,DPB1 ＊ 0402/＊ 0402. The HLA- Ⅱ genotype of HEL cells lines was as follow： DRB1 ＊ 1302/＊ 1302, DQB1 ＊ 0201/＊ 0201 ,DPB1 ＊ 1401/＊ 1401 and The HLA- Ⅱ genotype of MEG cells lines was as follow： DRB1 ＊ 1406/＊ 0410,DQB1 ＊ 0401/＊ 0401 ,DPB1 ＊ 0501/＊ 0501, respectively. [Conclusions] We studied the expression of HLA-Ⅱ genotype in myelogenous leukemia cell lines . The purpose of our study was to gain an increase understanding of HLA- Ⅱ genotype in myelogenous leukemia cell lines in order to study relationship between HLA-Ⅱ genotype and acute myelogenous leukemia. Further,this study is important for understanding immunogenetics of acute myelogenous leukemia in the clinic.

关 键 词：髓系白血病细胞系 HLA-Ⅱ基因型 聚合酶链反应-限制性片段长度多态性 

分 类 号：R733.7[医药卫生—肿瘤] R730.43[医药卫生—临床医学]