以HBc颗粒为呈现载体的真核表达载体pcDNA3.1-HBc^(NheI)的构建  

Use HBc as carrier to construct expression-vector PCDNA3.1-HBc^(Nhel)

作　　者：陈佳玉[1] 李景利 赵文静[3] 王海河[1] 李凡[1] 

机构地区：[1]吉林大学基础医学院病原生物实验室,吉林长春130021 [2]博泰生物技术有限公司,吉林长春130021 [3]北方肝胆病医院,吉林长春130062

出　　处：《中国现代医学杂志》2006年第1期54-56,共3页China Journal of Modern Medicine

摘　　要：目的构建含乙肝核心C基因的真核表达载体pcDNA3.1-HBcNheI,探索其作为DNA疫苗载体的可能性。方法采用分子克隆技术在原核表达质粒pTrc-core的HBcAgel-loop处添加NheI酶切位点,双酶切此重组载体pTrc-coreNheI及真核表达载体pcDNA3.1,产物经纯化、T4DNA连接酶连接,连接产物转化大肠杆菌DH5α,筛选抗Amp+克隆并提取质粒进行酶切及PCR鉴定。结果成功构建了真核表达质粒pcDNA3.1-HBcNheI。结论该重组质粒可作为治疗性及预防性疫苗的候选载体,为深入研究预防性及治疗性疫苗奠定了基础。[Objective] To construct DNA vaccine vector pcDNA3.1-HBc^Nhel. [Methods] We usedPCR to add the NheI site to plasmid pTrc-core, then HBc^Nhel gene was cloned into vector pcDNA3.1, the target genes were sequenced and the plasmids were extracted. [Results] We successfully constrct the DNA vaccine vector. [Conclusion] The plasmid pcDNA3.1-HBc^Nhel might be able to serve as curing or preventive DNA vaccines candidate.

关 键 词：DNA疫苗 免疫反应 HBc基因 

分 类 号：R392.7[医药卫生—免疫学]