手性整体式毛细管液相色谱柱的制备与应用  

Preparation and Application of Chiral Monolithic Column for Capillary Liquid Chromatography

作　　者：杨书雨[1] 陈令新[1] 姚波[1] 罗国安[1] 王义明[1] 

机构地区：[1]清华大学化学系,北京100084

出　　处：《分析测试学报》2006年第1期27-29,33,共4页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20475031;202990306);中国博士后科学基金资助项目(20040350303)

摘　　要：采用紫外光引发模式制备了甲基丙烯酸酯类毛细管整体柱,通过控制光照精确地控制了柱床的长度及边界。将环糊精(-βCD)共价连接到了高分子聚合物链上作为手性选择剂,在碱性环境下以液相色谱模式实现了在人体的代谢反应中有着重要作用的FITC标记的D,L-苯丙氨酸的分离,理论柱效125 000 p lates/m,并分析了流动相性质对分离的影响。Monolithic capillary columns based on polyacrylamides with UV initiated technology were made, and the length and boundaries of the column bed were made precisely through adjustment of UV radiation. β-cyclodextrin（β-CD） was bonded to the porous polymers of columns, which served as chiral selector. FITC labeled chiral amino acids, which had important impacts on metabonomics in vivo, were separated and detected by laser induced fluorescence（LIF） detection using liquid chromatography mode under an alkaline condition. Theoretical number of the column was about 125 000 plates/m. The influence of the mobile phase on the chromatographic performances was analyzed.

关 键 词：整体柱 原位聚合 Β-环糊精 激光诱导荧光 甲基丙烯酸酯 

分 类 号：O657.72[理学—分析化学]