脉冲电场上升沿对细胞跨膜电位的影响分析  

Effects of the Rising Edge of Pulsed Electric Field on the Transmembrane Voltage

作　　者：李成祥[1] 姚陈果[1] 孙才新[1] 米彦[1] 

机构地区：[1]重庆大学高电压与电工新技术教育部重点实验室,重庆400044

出　　处：《高电压技术》2006年第1期54-56,共3页High Voltage Engineering

基　　金：国家自然科学基金资助项目(50407007)

摘　　要：分析了电场生物效应实验和实用中脉冲电场上升沿的形成并基于经典球形单细胞模型推导出计及上升沿的脉冲电场所诱导的细胞跨膜电位的计算解析式。研究发现:脉冲电场上升沿与细胞跨膜电位峰值、波形密切相关,减少脉冲电场发生电路的电感来增大脉冲电场所诱导跨膜电位的峰值可提高电穿孔的效率。The pulses with rising edges are used in various experimental researches on biological effects in actual electric pulses. The reason why these rising edges exist is given. Analytic solution of transmembrane voltage in pulsed electric field with rising edge considered is derived using the classic singular cell model. From the analyses of the effects of rising edges on transmembrane voltage, it can be concluded that both the magnitude and waveform of the transmembrane voltage are closely related to the value of rising time constants. So the magnitude of transmembrane voltage can be increased and thus the efficiency of electroporation can be improved by reducing the inductance of pulsed electric field generation circuit.

关 键 词：脉冲电场 上升沿 跨膜电位 电穿孔 

分 类 号：Q274[生物学—细胞生物学]