2株具有亚硒酸盐还原能力细菌的筛选和鉴定  

Isolation and Identification of Two Strains With the Ability to Reduce Selenite

作　　者：王明义[1] 梁小兵[1] 朱建明[1] 郑娅萍[2] 魏中青[1] 赵由之[1] 

机构地区：[1]中国科学院地球化学研究所环境地球化学国家重点实验室,贵州贵阳550002 [2]贵阳医学院生物化学与分子生物学教研室,贵州贵阳550001

出　　处：《环境与健康杂志》2006年第1期31-32,共2页Journal of Environment and Health

基　　金：国家自然科学基金资助项目(40103007)

摘　　要：目的探讨具有还原亚硒酸盐能力的菌株在硒的生物地球化学转化过程中的作用。方法在湖北省恩施市双河乡渔塘坝采集富硒碳质泥岩样品,通过富基、分离和纯化培养,选择具有还原亚硒酸盐能力的菌株进行细菌鉴定和转硒效率的检测。结果从富硒碳质泥岩中筛选出2株具有还原亚硒酸盐能力的优势细菌,均鉴定为棒状杆菌属,亚硒酸钠转化率分别为3.9%,96.8%。结论棒状菌属在渔塘坝环境单质硒富集和硒的生物地球化学转化过程中起着重要的作用。Objective To screen out and isolate the strains that can reduce selenite in the high-Se environment of Yutangba. Methods The strains with the ability to reduce selenite were screened out by enrichment, isolation and purification culture. The strains were identified, the efficiency of transferring Se of the strains was measured. Results Two strains with the ability to reduce selenite were screened out from the soil and were preliminarily identified as Corynebacterium spp. The efficiency of transferring of one strain is 96.8%. Conclusion Corynebacterium spp. play an important role in the enrichment of Se and the process of Se biogeochemistry transformation in the environment of Yutangba.

关 键 词：亚硒酸钠 环境微生物学 棒状杆菌 渔塘坝 

分 类 号：R117[医药卫生—公共卫生与预防医学]