C57小鼠淋巴细胞瘤电转染GM-CSF基因研究  

Research of transferred GM-CSF gene into C57 mice T lymphoma with electroporation

作　　者：李瑞永[1] 蔡春玉[2] 金文昊[1] 张叔人[3] 

机构地区：[1]延边大学医院肿瘤科,吉林延吉133000 [2]延边大学医院呼吸科,吉林延吉133000 [3]中国医学科学院肿瘤研究所免疫室,北京100021

出　　处：《吉林医学》2006年第1期4-5,共2页Jilin Medical Journal

摘　　要：目的:制备高表达GM-CSF的C57小鼠T淋巴瘤细胞系。方法:将小鼠GM-CSF真核表达质粒用电穿孔方法导入C57小鼠T淋巴瘤细胞中,倍比稀释法制备单个细胞克隆,经RT-PCR、骨髓干细胞增殖实验和集落形成实验筛选相对高表达GM-CSF的RMA-GM克隆。结果:获得了高表达GM-CSF的RMA细胞系RMA-GM,其出瘤时间延长。结论:C57小鼠RMA-GM克隆细胞出瘤时间延长,肿瘤细胞免疫原性增强。Objective To make high GM-CSF expressing C57 mice lymphoma cell lineage. Method The eukaryotic GM-CSF expressing plasmid was transfected into C57 mice T lymphoma cell by electroporation, A relative GM-CSF high expressing cell clone was selected by RT-PCR, haemopoietic stem cell proliferative assay and hematopoietic progenitor cell colony for marion assay after screeing by G-418 and cloning by coubling dilution, Results A GM-CSF high expressing cell lineage RMA-GM was selected whose ability of tumor formation time was slow than RMA. Conclusion The tumor formation time of RMA-GM is prolong and tumor original immunity is strengthen in vivo.

关 键 词：淋巴瘤 电转染 基因 抑制 肿瘤 质粒 小鼠 

分 类 号：R733.1[医药卫生—肿瘤]