棒状杆菌亮氨酸操縱子在大肠杆菌中的克隆和表达  

Cloning and Expression of Leucine Operon from Corynebacterium Glutamicum in Escherichia Coli

作　　者：温晋[1] 周天鸿[1] 郑兆鑫[2] 严维耀[2] 

机构地区：[1]暨南大学生物学系 [2]复旦大学遗传研究所

出　　处：《暨南大学学报（自然科学与医学版）》1989年第1期41-45,共5页Journal of Jinan University(Natural Science & Medicine Edition)

摘　　要：以质粒pTZ22为载体,用HindШ限制酶切割谷氨酸棒状杆菌染色体DNA进行克隆。分离到带有亮氨酸操纵子的4.5kb片段。用Southern切口移位分子杂交检测,证明在4.5kb的片段中既包含有leuB基因,而且对leuA基因也表现同源性。Using Hindlll digested plasmid pTZ22 as a vector, a 4.5 kb fragment of leu operon was cloned from chromosomal DNA of Corynebacterium glutamicum。It was analysed by Southern hybridizalion and confirmed that the 4.5kb fragment not only containned leuB gene but also contained homology with leuA gene。

关 键 词：基因克隆 亮氨酸操纵子 棒杆菌 

分 类 号：Q939.118[生物学—微生物学]