水稻苯达松敏感致死基因的RAPD标记的克隆及测序  

Cloning and Sequence of RAPD Marker Linked to the Bentazon Susceptible Lethality Gene in Rice

作　　者：彭凌[1] 朱必凤[1] 刘主[1] 刘盈盈[2] 

机构地区：[1]韶关学院英东生物工程学院,广东韶关512005 [2]南昌大学生命科学学院,江西南昌330047

出　　处：《韶关学院学报》2005年第12期78-81,共4页Journal of Shaoguan University

基　　金：广东省自然科学基金资助项目(001446)

摘　　要：用3S Spin DNA Agarose Gel Purification Kit试剂盒将与水稻苯达松敏感致死基因相连锁的RAPD遗传标记S20-420和S316-600回收纯化,连接于pGEM-T载体并克隆测序,得到了S20-420和S316-600的全序列,其长度分别为423 bp6、06 bp.将两端序列设计特异PCR扩增引物可用于检测水稻苯达松敏感致死基因和标记辅助育种.Randomly amplified polymorphic DNA（RAPD） was employed to detect molecular markers linked to the bentazon susceptible lethality gene in rice. RAPD marker S20 - 420 and S316 - 600 were linked to the bentazon susceptible lethality gene in rice. S20 - 420 and S316 - 600 fragment were reclaimed by 3S Spin DNA Agarose Gel Purification Kit and cloned in T - easy vector. Sequencing finally showed that the S20 - 420 fragment was actually 423 bp in lenth, the S316 - 600 fragment was actually 606 bp. It is suggested that the sequence of RAPD markers might be used as a basis for synthesizing the specific PCR primers and for detecting the bentazon susceptible lethality gene in rice and molecular marker assisted selection（MAS）.

关 键 词：水稻 苯达松敏感致死基因 RAPD 克隆 测序 

分 类 号：S511[农业科学—作物学]