GSTT1、GSTM1、NQO1、RAD51和XRCC3基因多态性与慢性粒细胞白血病发生关系的研究  

Relationship between GSTT1,GSTM1,NQO1,RAD51 and XRCC3 genes polymorphisms and chronic myeloid leukemia

作　　者：杨琳[1] 徐世才[1] 刘旭平[1] 张美荣[1] 张悦[1] 肖志坚[1] 

机构地区：[1]中国医学科学院中国协和医科大学血液学研究所,血液病医院

出　　处：《国际输血及血液学杂志》2006年第1期2-5,共4页International Journal of Blood Transfusion and Hematology

基　　金：国家自然科学基金(30270573)

摘　　要：目的探讨GSTT1、GSTM1、NQO1、RAD51和XRCC3基因多态性与我国慢性粒细胞白血病(CML)发生遗传易感之间的关系。方法共120例CML患者和458名与患者无血缘关系的正常人,用多重PCR方法检测GSTT1和GSTM1基因型,用PCR-RFLP方法分析RAD51,XRCC3,NQO1基因型。结果CML患者GSTT1和GSTM1缺失型比例分别为50.8%和59.2%,与正常对照组无显著差异(分别为42.8%和53.1%)。CML患者NQO1C/T和T/T基因型的比例(60.0%)、RAD51G135CG/C基因型比例(26.9%)和XRCC3-241Met杂合子缺失型(Thr/Met)的比例(9.2%)均与正常对照组(分别为65.3%,12.4%和9.2%)无统计学差异。结论本研究结果提示GSTT1、GSTM1、NQO1、RAD51和XRCC3基因型与我国CML的发生无显著相关性。Objective To investigate the impact of GSTM1, GSTT1, NQO1, RAD51 and XRCC3 genotypes on the chronic myeloid leukemia （CML） susceptibility. Methods GSTT1, GSTM1, NQO1, RAD51 and XRCC3 genotypes were detected in 120 patients with de novo CML and 458 controls by PCR or PCR-RFLP. Results There were no significant difference of the incidence of GSTT1 null genotype and GSTM1 null genotype between CML patients（50.8% and 59.2%, respectively） and controls（42.8% and 53.1%, respectively）. The frequency of NQO1 C/T and T/T genotypes （60.0%）, RAD51 G135CG/C genotype （26.9%） and XRCC3-241 Thr/Met genotype （9.2%）of CML patients were also no significant difference between CML patients and controls（65.3%, 12.4% and 9.2%, respectively）. Conclusion Our results suggested that GSTM1, GSTT1, NQO1, RAD51 and XRCC3 genotypes mightn＇t predicate highrisk individuals for CML.

关 键 词：慢性粒细胞白血病 遗传易感 GSTT1/M1基因 NQO1基因 RAD51基因 XRCC3基因 

分 类 号：R733.7[医药卫生—肿瘤]