猪源致病性沙门氏菌耐药基因的分析  

Detection of Antimicrobial Resistance Genes of Pathogenic Salmonella from Swine

作　　者：马孟根[1] 王红宁[2] 余勇[3] 李成忠[2] 张东[3] 羊云飞[2] 刘世贵[1] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]四川农业大学动物科技学院,雅安625014 [3]四川省动物防疫监督总站,成都610041

出　　处：《畜牧兽医学报》2006年第1期65-70,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家"十五"重点科技攻关子课题(2002BA514A-12-5)

摘　　要：采用平板稀释法,选用氨基糖苷类、四环素类、磺胺类和氯霉素类4大类抗生素的11种药物,对30株猪源致病性沙门氏菌进行了药敏试验,结果有28株菌(93.3%)至少对一种药物有耐药性;对四环素、强力霉素、磺胺甲基异口恶唑、复方新诺明、链霉素、卡那霉素和氯霉素有耐药性的菌株较普遍,在所有菌株中占比例分别为83.3%、80%、80%、76.7%、60%、56.7%和56.7%。设计了25对引物,对耐药基因进行了扩增及序列测定,结果扩增到13种耐药基因,与GenBank中的相应基因有很高的同源性(≥98.1%)。30株猪源致病性沙门氏菌中至少含有一种耐药基因的菌株有28株(93.3%),sulⅠ、aph(3′)-Ⅱat、etC、Cat1t、etA和aadA1耐药基因较为普遍,检出率分别为76.7%、60%、60%、43.3%、40%和36.7%。药敏试验结果与耐药基因检测结果有很高的一致性(≥88%)。Antimicrobial MICs of 30 pathogenic salmonella isolates from swine were determined by using plate dilution method. 11 antibiotics （belonging to aminoglycosides, tetracyclines, sulfonamides and chloramphenicols） were used. The results showed that twenty-eight strains （93.3%） of these salmonella isolates were resistant to at least one antimicrobial agent; resistance to the following antibiotics was common., tetracycline （83. 3 % ）, doxycycline （80 % ）, sulfamethoxazole （80%）, sulfamethoxazole timethoprim （76.7%）, streptomycin （60%）, kanamycin （56.7%） and chloramphenieol （56.7 %）. 25 pairs of primers were designed and antimicrobial resistance genes were amplified, sequenced and analyzed. A total of 13 different antimicrobial resistance genes conferring resistance to four categories of antimicrobials were identifed and the homologous rate of each gene was very high compared with the correspondence gene in GenBank （≥98.1%）. At least one antimicrobial resistance gene was detected in 28 pathogenic salmonella isolates, accounting for 93.3% （28/30）. The following antimicrobial resistance genes were commonly present： sulI （76.7%）, aph（3′）-Ⅱa （60%）, tetC （60%）, Catl （43.3%）, tetA （40%） and aadA1（36.7%）. Antimierobial phenotypes results were very consistent with the antimicrobial resistance genes （≥88%）.

关 键 词：沙门氏菌 耐药基因 检测 

分 类 号：S852.612[农业科学—基础兽医学]