固定化青霉X5对孔雀石绿的脱色研究  

Study on decolorization of malachite green by immobilized Penicillinm X5

作　　者：董新姣[1] 谢荣敏[1] 

机构地区：[1]温州师范学院生命与环境科学学院,温州325027

出　　处：《环境污染治理技术与设备》2006年第1期45-49,共5页Techniques and Equipment for Environmental Pollution Control

基　　金：温州市科技计划项目(N2001A48)

摘　　要：采用植物载体玉米芯对青霉X5进行固定化。利用正交实验,确定了固定化最优操作条件为:菌量0.4 g/L,载体大小为1/2×π×12×1 cm3,载体个数为10,摇床转速为100 r/m in。同时考察了各种因素如温度、pH、碳源浓度和盐浓度等对染料脱色的影响,结果表明,在温度为30℃、pH 4.0、碳源浓度为10 g/L条件下,以100 r/m in的摇速培养,脱色效果最佳,脱色率达96.1%。重复脱色实验表明,固定化青霉X5经过4次重复利用后,固定化细胞的结构仍良好,脱色率仍在90%以上。脱色平衡时间为360 m in。The cell-adsorption of reactive brilliant blue KN-R immobilized by new kinds of plant-carrier, corn bar, was studied. Optimal preparation condition of the strain immobilization is determined by the cross-test experiments. The result indicated that optimal preparation for immobilization could be obtained at the following conditions： mycelium amount O. 4 g/L , 1/2 × π × 12 ×1 cm3 in carrier size, 10 in carrier number and 100 r/min for the rate of shaker. The factors affecting decolorization capability on 10 mg/L malachite green by immobilized Penicillinm sp. , such as temperature, pH,carbon source concentration,salinity were examined. The result showed that under optimum decolorization condition （30℃ , pH 4.0, 10 g/L） , the capability of decolorization was the best. After decolorizing of four times, the structure of immobilized cell remained the same, and decolorization rate was still above 90%. The equilibrium time of decolorization is 360 min.

关 键 词：青霉菌 固定化 植物载体 孔雀石绿 脱色 

分 类 号：X173[环境科学与工程—环境科学]