白细胞介素-18修饰的树突状细胞瘤苗的制备及其抗肿瘤免疫保护作用的研究  

作　　者：王峻[1] 何炳荣[1] 孙宏高[1] 邵传森[2] 沈建根[2] 

机构地区：[1]杭州市红十字会医院,310003 [2]浙江大学免疫学研究所,310031

出　　处：《浙江临床医学》2006年第1期5-6,共2页Zhejiang Clinical Medical Journal

摘　　要：目的研究树突状细胞(DC)瘤苗的抗肿瘤免疫保护治疗作用,进一步探索DC为基础的肿瘤免疫治疗的新途径。方法以腺病毒为载体转染IL-18和/或gp100-DC,制备DC瘤苗,以ELISA、RT-PCR、FACS及混合淋巴细胞反应(MLR)检测所制备的DC瘤苗的效果,以黑色素肿瘤动物模型鉴定DC瘤苗的抗肿瘤免疫保护作用。结果本研究成功制备了DC瘤苗,IL-18/gp100-DC的抗肿瘤免疫保护作用明显强于IL-18-DC或gp100-DC瘤苗。结论Th1型细胞因子IL-18和gp100共转染能显著增强DC的抗肿瘤作用。Objective To explore the anti - tumor effect of dendritic cells （DC） vaccine and new immunotherapy method. Methods Cytokine interleukin - 18 （IL- 18） an melanoma- associated antigen gpl00 were transfectod into bone marrow- derived DC （IL- 18/gp100- DC）, which were used as vaccine to induce the protective in a B16 melanoma model. And the effect of DC vaccine were detected through ELISA, RT - PCR, FACS and MLR. Results DC vaccines were successful constructed. The effect of anti - tumor immunity in IL - 18/gp100 - DC was significant higher than that of IL - 18 - DC or gp100 - DC. Conclusion The effect of anati - tumor was significanly enhanced with DC vaccine co - expressing Th1 cytokine IL- 18 and gp100.

关 键 词：树突状细胞瘤苗 免疫保护作用 抗肿瘤免疫 白细胞介素-18 制备 混合淋巴细胞反应 TH1型细胞因子 IL-18 DC瘤苗 

分 类 号：R73-36[医药卫生—肿瘤]