Detection and Characterization of Non-covalent Complex between Lappaconitine and β-Cyclodextrin by Electrospray Ionization Tandem Mass Spectrometry  

作　　者：Qing Xuan XU Li LI Hao YUE Zhi Qiang LIU Ming Quart GUO Shu Ying LIU 

机构地区：[1]Laboratory of New Drug Research and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences Changchun 130022

出　　处：《Chinese Chemical Letters》2006年第1期65-68,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.30472134,20173057);the State Great Basic Research Project of China(No.2003CCA03100);the Great Research Project of Chinese Academy of Sciences(No.KGCX2-SW-213-06).

摘　　要：The non-covalent complexes between lappaconitine （LA） and β-cyclodextrin （β-CD） have been detected and characterized by electrospray ionization combined with ion trap tandem mass spectrometry （ESI-MSn）. The experimental results showed that only 1：1 non-covalent complex can be formed in different starting molar ratios of LA to β-CD. Furthermore, the diagnostic fragmentation of the β-CD-LA complex, with a significant contribution of covalent fragmentation of LA leaving the N-acetyl anthranoyl （AN） moiety inserted to β-CD, provided the convincing evidence for the formation of non-covalent complex between LA and β-CD and the cite of LA molecule included to cavity of β-CD assigned to AN residue.The non-covalent complexes between lappaconitine （LA） and β-cyclodextrin （β-CD） have been detected and characterized by electrospray ionization combined with ion trap tandem mass spectrometry （ESI-MSn）. The experimental results showed that only 1：1 non-covalent complex can be formed in different starting molar ratios of LA to β-CD. Furthermore, the diagnostic fragmentation of the β-CD-LA complex, with a significant contribution of covalent fragmentation of LA leaving the N-acetyl anthranoyl （AN） moiety inserted to β-CD, provided the convincing evidence for the formation of non-covalent complex between LA and β-CD and the cite of LA molecule included to cavity of β-CD assigned to AN residue.

关 键 词：LAPPACONITINE Β-CYCLODEXTRIN non-covalent complex ESI-MS CID. 

分 类 号：R979.1[医药卫生—药品] R917[医药卫生—药学]