柱层析法纯化变形链球菌表面蛋白质抗原Ⅰ/Ⅱ的研究  

Purification and characterization of surface protein antigen I / I from Streptococcus mutans by column chromatography

作　　者：杜民权[1] 樊明文[1] 边专[1] 张平[1] 

机构地区：[1]湖北医科大学口腔医学院,武汉市430070

出　　处：《口腔医学纵横》1996年第3期131-134,共4页Journal of Comprehensive Stomatology

基　　金：国家自然科学基金(39270720号)

摘　　要：用硫酸铵沉淀、阴离子交换柱层析、凝胶过滤柱层析从变形链球菌培养上清液中提取、纯化了表面蛋白质抗原Ⅰ/Ⅱ(简称抗原Ⅰ/Ⅱ),该抗原能与特异性抗抗原Ⅰ/Ⅱ血清发生免疫学沉淀反应;SDS-PAGE显示纯抗原Ⅰ/Ⅱ为单一蛋白区带,分子量为190546 d;Lowry法测定蛋白质含量为0.8mg/ml;氨基酸组成分析表明丙氨酸、天冬氨酸、谷氨酸、赖氨酸含量较多,酸性氨基酸占24.1％,碱性氨基酸占11.4％,表明蛋白质的等电点偏低。Surface protein antigen I / II (antigen I / I ) was extracted and purified from Streptococcus mutans Ingbritt culture supernatant by 65% saturated ammonium sulphate precipitation. ion exchange column chromatography and gel filtration. The antiggen reacted with specific anti-antigen I / I serum by immunodiffusion. SDS-PAGE of purified antigen I / II exhibited a single band, with weight of 190546d. The protein concentration was 0. 8mg/ ml estimated by Lowry method. Amino acids composition revealed acidic anino acid residues contain 24. 1%, basic amino acid residues contain 11.4%.

关 键 词：变形链球菌 表面蛋白质抗原Ⅰ/Ⅱ 柱层析 纯化 

分 类 号：R780.2[医药卫生—口腔医学] R392.11[医药卫生—临床医学]