用多孔微载体Cytopore高密度培养CHO工程细胞和两株杂交瘤细胞  

High density cultivation of recombinant CHO cell line and two strains of hybridomas with porous microcarrier cytopore

作　　者：肖成祖[1] 黄子才[1] 李文青[1] 屈文禄 高丽华[1] 刘高艳 

机构地区：[1]军事医学科学院生物工程研究所

出　　处：《军事医学科学院院刊》1996年第3期191-194,共4页Bulletin of the Academy of Military Medical Sciences

摘　　要：采用Ｃｙｔｏｐｏｒｅ多孔微载体和低血清添加剂，在１．５ＬＣｅｌｉｇｅｎ灌流培养系统中成功地培养了重组ＣＨＯ工程细胞ＣＬ－１１Ｇ和产尿激酶原单抗的杂交瘤细胞。细胞密度均超过１×ｌ０７／ｍｌ，尿激酶原和抗体的产量均随细胞的密度增加而增加。Using porous microcarrier Cytopore and low serum medium supplement, a recombinant CHO cell line CL 11G and two strains of hybridomas producing prourokinase monoclonal antibody have been successfully cultivated. The density of these cells could exceed 1×10 7 cells/ml. The yields of prourokinase and monoclonal antibody were enhanced with increasing of cell density. So this porous microcarrier was suitable for large scale cultivation of recombinant CHO cells and hybridomas.

关 键 词：CHO细胞 杂交瘤细胞 尿激酶原 单克隆抗体 

分 类 号：R394.8[医药卫生—医学遗传学] R329-33[医药卫生—基础医学]