三种丙型肝炎病毒核蛋白在人肝癌细胞的表达  

Gene expression profiles on three kinds of genotype hepatitis C virus core protein in Huh-7 cell line with microarray analysis

作　　者：窦骏[1] 王净[2] 刘蓬勃 张兴建 

机构地区：[1]东南大学基础医学院病原生物学与免疫学系,南京210009 [2]东南大学附属中大医院妇产科 [3]Emory Universiey School of Medicine,U.S.A. [4]Harvard Medical School,U.S.A.

出　　处：《中华预防医学杂志》2006年第1期38-41,共4页Chinese Journal of Preventive Medicine

摘　　要：目的用基因芯片分析丙型肝炎病毒(HCV)三种基因型HCV-1b、HCV-2a和HCV-4d核蛋白在人肝癌细胞系(Huh-7)的基因表达,重新认识HCV核蛋白功能及其致病机制。方法建立表达HCV三种基因型核蛋白的Huh-7细胞系,提取总RNA制备探针,与Affymetrix人基因芯片HG-U133杂交。对基因表达改变≥3或1.5倍的基因,通过Affymetrix网站用NetAffx进一步分析,并按生物学功能分类。结果HCV-1b核蛋白引起16个基因表达改变,其中免疫反应基因PF4V1和SPP1分别上调3.4和4.4倍;HCV-2a核蛋白对免疫反应基因CXCL5和凋亡基因BTF分别下调3.4和3.1倍,但对凋亡基因HRK、LZTS1则能上调3.2和3.4倍;与HCV-1b和HCV-2a核蛋白相比,HCV-4d核蛋白可引起111个基因表达改变,对基因表达谱有更明显的影响。若设定基因表达改变≥1.5倍有意义,HCV-1b和HCV-2a核蛋白可引起若干相同基因的改变。结论HCV三种基因型核蛋白引起的基因表达谱各有特点,主要涉及免疫反应、凋亡、信号传导等基因,这对重新认识HCV核蛋白功能及致病机制均有重要意义。Objective To analyze three kinds of genotype hepatitis C virus （HCV） core protein expressed in human hepatoma （Huh-7） cell line and to recognize HCV core proteins biological function and its pathogenic mechanism. Methods The Huh-7 cell expressed three kinds of core proteins were established respectively. Affymetrix human gene chip was used for identifying the gene expression dependently on Affymetrix＇ s protocol. All genes changed by 3 or 1.5 folds between the transfected cells and a control cells were further analyzed, and annotated by using NetAffx analysis through Affymetrix website and were categorized based on their hiological processes. Results The HCV-lb core protein caused 16 genes up/down-regulated expression, of which the immune response genes of PF4V1 and SPP1 were up-regulated 3.4 or 4. 4 folds respectively. The HCV-2a core protein had caused the immune response gene CXCL5 and apoptosis gene BTF a down-regnlated expression of 3.4 and 3. 1 folds respectively, but caused the apoptosis genes of HRK and LZTS1 an up-regulated expression of 3.2 and 3.4 folds respectively. As compared with HCV 1b or 2a core protein, HCV-4b core protein caused 111 genes expression changing and it had more obvious effects on gene expression. If we applied 1.5 fold change for a comparison gene expression, a few of the same gene expression profiles might be caused by these two core proteins. Conclusion The three kinds of HCV core protein should have its own expression character and be mainly shown in immune responses, signal transduction, apeptosis, etc. It should be helpful for our recognizing the HCV core protein biological function and its pathogenic mechanism.

关 键 词：C型肝炎样病毒属 核蛋白类 基因表达 

分 类 号：R735.7[医药卫生—肿瘤] R511[医药卫生—临床医学]