浙江蝮蛇蛇毒诱导人白血病K562细胞凋亡  

Apoptosis of human leukemia K562 cells induced by snake venom of Agkistrodon Halys Pallas in Zhejiang Province

作　　者：董庆华[1] 郑树[1] 吕庆华[1] 何立明[1] 

机构地区：[1]浙江大学医学院附属第二医院肿瘤研究所,杭州310009

出　　处：《中华急诊医学杂志》2006年第2期121-123,共3页Chinese Journal of Emergency Medicine

基　　金：浙江省科技厅资助项目(2002D3007);浙江省卫生厅资助项目(2003B038)

摘　　要：目的研究浙江蝮蛇毒诱导人白血病K562细胞凋亡的作用及机制。方法MTT法测IC50值及细胞毒作用,Hoechest33258荧光染色法观察凋亡小体形成,流式细胞仪PI染色法检测凋亡峰和细胞周期,同时FCM及Westernblot法检测Bcl2蛋白表达。结果浙江蝮蛇毒能抑制细胞生长且呈剂量依赖关系,并能诱导人白血病K562细胞凋亡,Bcl2蛋白表达下降。结论浙江蝮蛇毒能诱导人白血病K562细胞凋亡且呈剂量依赖关系,此作用与Bcl2蛋白表达下调有关。Objective To study the apoptosis of human leukemia K562 cells induced by snake venom of Agkistrodon Halys Pallas in Zhejiang Province. Methods ICs0 value and cytotoxity of K562 cell were detected by MTT method. Apoptotic cells were dyed by Hoechest 33258. Sub-G1 peak and cell cycle were detected by FCM. Protein expression of Bcl-2 gene was detected by FCM and western-blot method. Results The snake venom of Agkistrodon Halys Pallas in Zhejiang Province inhibited the growth of K562 cells, which appeared dose-dependent. The snake venom induced apoptosis of K562 cells. Meanwhile, protein expression of Bcl-2 was down-regulated. Conclusion Snake venom of Agkistrodon Halys Pallas in Zhejiang could induce apoptosis of human leukemia K562 cells. The mechanism may be related to downregulation of Bcl-2 gene expression.

关 键 词：浙江 蝮蛇蛇毒 白血病 B细胞淋巴瘤 白血病-2基因 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤]