农药久效磷分子印迹聚合物合成及其亲合性评估  

PREPARATION AND AFFINITY ASSESSMENT OF MONOCROTOPHOS MOLECULARLY IMPRINTED POLYMERS

作　　者：严守雷[1] 高志贤[1] 房彦军[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《高分子学报》2006年第1期160-163,共4页Acta Polymerica Sinica

基　　金：国家自然科学基金(基金号30371218);国家863青年基金(基金号2004AA649110)资助项目

摘　　要：Uniformly imprinted polymer microspheres were prepared by precipitation polymerization in chloroform.MCP（monocrotophos）,MAA（methacrylic acid）,EGDMA（ethylene glycol dimethacrylate） and AIBN（azobisisobutyronitrile） were used as template,functional monomer,crosslinker and initiator,respectively.UV and IR spectra were used to demonstrate the mechanism of the interaction between MCP and MAA.It was found that one MCP was entrapped by three MAA molecules in chloroform.Langmuir model was used to fit the adsorption data,the results showed that the MCP imprinted polymer microspheres showed specific binding sites to the MCP.The data obtained in equilibrium adsorption experiments were processed by Scatchard analysis,the dissociation contents(KD1) and the apparent maximum binding capacity (Bmax1) were KD1=0.062?mmol/L,Bmax1=3.26?μmol/g for high affinity binding sites and KD2=3.03?mmol/L,Bmax2=65.70?μmol/g for low affinity binding sites,respectively.The MCP imprinted polymers have showed higher affinity to MCP than to the other organophosphorus pesticide used.So the imprinted polymers microspheres can be used for trace pesticide residue separation,enrichment and analysis.Unit＇ormly imprinted polymer microspheres were prepared by precipitation polymerization in chloroform MCP （ monocrotophos ）, MAA （ methacrylic acid ）, EGDMA （ ethylene glycol dimethacrylate ） and AIBN （azobisisobutyronitrile） were used as template, functional monomer, cross-linker and initiator, respectively. UV and IR spectra were used to demonstrate the mechanism of the interaction between MCP and MAA . It was found that one MCP was entrapped by three MAA molecules in chloroform. Langmuir model was used to fit the adsorption data, the results showed that the MCP imprinted polymer microspheres showed specific binding sites to the MCP. The data obtained in equilibrium adsorption experiments were processed （ KDI ） and the apparent maximum binding capacity （ Bmaxl ） were by Scatchard analysis, the dissociation contents KD1= 0.062 mmol/L, Bmaxl = 3.26 μmol/g for high affinity binding sites and KD2= 3.03 mmol/L, Bmax2= 65.70 μmol/g for low affinity binding sites, respectively. The MCP imprinted polymers have showed higher affinity to MCP than to the other organophosphorus pesticide used. So the imprinted polymers microspheres can be used for trace pesticide residue separation, enrichment and analysis.

关 键 词：分子印迹 久效磷 沉淀聚合 农药 机磷农药 

分 类 号：TQ450.1[化学工程—农药化工]