血清蛋白质谱模型对胃腺癌诊断的应用性研究  

Application of Serum Proteomic Patterns for Detection of Gastric Adenocarcinoma

作　　者：梁勇[1] 刘池波[1] 李继承[2] 

机构地区：[1]台州学院附属市立医院,浙江台州318000 [2]浙江大学细胞生物学研究所,浙江杭州310031

出　　处：《中国肿瘤》2006年第2期127-130,共4页China Cancer

基　　金：浙江省教育厅科研项目(20050060)

摘　　要：[目的]探讨用蛋白质芯片技术筛选胃腺癌患者血清蛋白质表达谱,寻找血清中的标志性蛋白。[方法]采用蛋白质生物芯片表面增强激光解析电离飞行时间质谱(SELDI)技术,运用SAX2(StrongAnionicExchanger)蛋白质芯片检测胃腺癌患者、胃炎患者和健康者血清,建立诊断模型,然后进行单盲模型验证。[结果]发现5910Da、5084Da和8691Da的三个蛋白质荷比峰(M/Z)在胃腺癌和健康组比较中具有显著性差异。5910Da、6440Da的两个蛋白质荷比峰(M/Z)在胃腺癌和胃炎组中比较具有显著性差异。[结论]建立了胃腺癌的血清蛋白指纹质谱,为以后的胃癌蛋白质组学研究奠定了一定的基础,建立了以5910Da、5084Da、8691Da和6440Da四个蛋白质峰为模型区分胃腺癌与非胃腺癌的血清蛋白表达质谱诊断模型,为胃腺癌的临床诊断提供了一条崭新的途径和方法。[Purpose]To screen out serum protein profiling of astric adenocarcinoma by surface-enhancedlaser desorption/ionization time of fligh mass spectrometry （SELDI-TOF-MS）to discovering the discriminalory proteins.[Methods]The diagnosis model was established by SELDI technique with SAX2 chip to detect the serum of patients with gastric adenocarcinoma, gastricism and healthy persons, and tested by single-blind model.[Results]Three specific protein fingerprint biomarkers massed at 5910,5084,8691 between gastric adenocarcinoma group and control group with statistical significance. Two specific protein fingerprint biomarkers massed at 5910,6440 between gastric adenocarcinoma group and gastricism group with statistical significance.[Conclusions] A gastric adenocarcinoma protein fingerprint and a foundation for later study of gastric cancer proteomics is developed. The diagnostic model of the protein profiling（5910Da,5084Da,8691Da,6440Da） for distinguishing gastric adenocarcinoma from non-gastric adenocarcinoma is established to aid a new approach in clinical diagnosis for gastric adenocarcinoma.

关 键 词：表面增强激光解析电离-飞行时间质谱 蛋白质芯片 生物标记 胃腺癌 

分 类 号：R735.2[医药卫生—肿瘤]