七叶皂甙钠和乌司他丁联合治疗急性胰腺炎的实验研究  

Experimental study on combined use of sodium aescinate and ulinastatin in treatment of acute pancreatitis in rats

作　　者：陈中华[1] 周华佳[2] 顾海峰[1] 

机构地区：[1]浙江大学医学院附属第一医院,浙江杭州310003 [2]浙江大学医学院附属妇产科医院检验科,浙江杭州310006

出　　处：《中国中西医结合急救杂志》2006年第1期51-53,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

摘　　要：目的:观察七叶皂甙钠联合乌司他丁对大鼠急性胰腺炎(AP)的治疗效果。方法:75只雄性SD大鼠随机分为正常组、AP组、七叶皂甙钠组(SA组)、乌司他丁组(U组)和七叶皂甙钠联合乌司他丁治疗组(SA+U组)。AP、SA、U和SA+U组大鼠制模,SA、U和SA+U组给予相应药物治疗。观察各组血淀粉酶、脂肪酶、肿瘤坏死因子α(TNFα)、丙二醛(M DA)和超氧化物歧化酶(SOD)水平,并观察胰腺病理改变;测定胰腺湿重/干重比。结果:AP组SOD明显下降,其余各指标均明显升高(P<0.05或P<0.01),胰腺病理性损伤较重。与AP组比较,SA和U组血淀粉酶、脂肪酶、TNFα、病理学评分均降低,细胞超微结构损伤减轻,胰腺湿重/干重比增加,M DA降低,SOD升高,差异均有显著性(P<0.05或P<0.01)。SA+U组上述指标改善较SA组和U组更明显,差异有显著性(P均<0.05)。结论:七叶皂甙钠和乌司他丁联用治疗急性胰腺炎时两者间可能存在协同作用,疗效较分别单独使用明显增强。Objective ： To observe the therapeutic ettects ot sodium aesclnate anct uilnastatln in treatment of acute pancreatitis （AP） of rats. Methods： Seventy - five male Spraque - Dawely （SD） rats were randomly divided into normal group, AP group, sodium aescinate group （SA group）, ulinastatin group （U group） and sodium aescinate combined with ulinastatin group （SA＋U group）. Plasma concentrations of amylase and lipase, tumor necrosis factor-α （TNF-α）, ratio of pancreatic wet weight to dry weight （W/D）, blood level of superoxide dismutase （SOD） and malondialdehyde （MDA） were determined. The pathological changes of pancreas were studied under light and electron microscopes. Results： SOD levels decreased and the concentrations of amylase, lipase, TNF -α, MDA level and W/D pancreas increased （P〈0.05 or P〈0.01） in AP rats, while the pathological change of pancreas was severe. Compared with AP group, the plasma concentrations of amylase and lipase, TNF-α, the pathological scores and MDA in SA group and U group decreased, while SOD was increased and ultrastructural injury was markedly attenuated, the ratio of W/D was increased respectively （all P〈0.05）, but the indexes were decreased more significantly in SA＋U group （all P〈0.05）. Conclusion： Ulinastatin combined with sodium aescinate is more effective than either of them used alone in treatment of severe AP.

关 键 词：七叶皂甙钠 乌司他丁 胰腺炎 急性 

分 类 号：R576[医药卫生—消化系统]