泥蚶提取物抗肿瘤作用的实验研究  

Antitumor effects of extractive from Arca Granosa Linnaeus

作　　者：王贞丽[1] 初晓[2] 杨秀 陈守国 王春波[4] 

机构地区：[1]济南军区青岛第一疗养院,山东青岛266071 [2]青岛市立医院 [3]青岛海生肿瘤医院 [4]青岛大学医学院

出　　处：《山东医药》2006年第3期15-17,共3页Shandong Medical Journal

基　　金：国家火炬计划资助项目(2005EB021065)

摘　　要：目的 探讨泥蚶提取物——海生素抗肿瘤的作用。方法 采用四氮唑盐衍生物（XTT）比色法观察体外培养的肿瘤细胞株Ketr-3和A549，细胞增殖情况，测定细胞蛋白含量和周期分布。计算小鼠移植性S180瘤和艾氏腹水瘤（EAc）的抑瘤率和小鼠生存时间及免疫功能。结果 海生素对Kerr-3和A549细胞具有明显的抑制作用，分别阻滞在细胞G0～G1期和G2～M期。对小鼠S180瘤的抑瘤率达到19．34％～55．19％，明显延长EAC小鼠的生存时间，结论 海生素对不同肿瘤细胞的抑制作用机制可能不同，对移植性肿瘤抑制作用与其免疫功能提高有关。Objective： To investigate the antitumor effect of haishengsu, an extractive from arca granosa linnaeus, in vitro and in vivo. Methods ： The proliferation of the cultured tumor cell lines of Kerr-3 and A549 were evaluated by XTT method. The protein concentration of the cells and the cell cycle distribution were calculated. Transplanted tumor models of S180 and ehrlich ascites carcinoma （EAC） in mice were used to evaluate the antitumor effect of the extractive. Results： The inhibition rates on Ketr-3 and A549 cells growth were directly proportional to haishengsu concentrations. It caused a significant G0～G1, G2～M phase arrest in Ketr-3 and A549 cells respectively. Haishengsu injection could inhibit the Sis0 by 19.34%～55.19% and significantly prolong the life span of EAC-bearing mice. Conclusion： Haishengsu could inhibit tumor and the mechanisms might be both inhibiting the growth of tumor cells directly and strengthening immune system.

关 键 词：海生素 泥蚶 抗肿瘤 四氮唑盐衍生物 免疫功能 

分 类 号：R73-36[医药卫生—肿瘤] R979.1[医药卫生—临床医学]