丹参脂溶性成分的ESI-MS行为及其特征图谱研究  

EIS-MS Performance and fingerprint of lipophilic constituents from Radix Salviae Miltiorrhiza

作　　者：韩凤梅[1] 张玲[1] 陈怀侠[1] 陈勇[1] 

机构地区：[1]湖北大学中药生物技术省级重点实验室,湖北武汉430062

出　　处：《中草药》2006年第1期122-125,共4页Chinese Traditional and Herbal Drugs

基　　金：科技部"十五"攻关项目(2001BA701A01);湖北省青年杰出人才科学基金项目(2002AC004)

摘　　要：目的研究脂溶性丹参酮类成分的ES I-M S规律,建立丹参药材脂溶性丹参酮类成分提取物ES I-ITM S特征图谱。方法应用电喷雾离子阱质谱(ES I-ITM S)技术研究丹参酮类成分的ES I-M S规律;用95%乙醇超声提取丹参药材中丹参酮类物质,在正离子方式检测模式下,直接进样应用一级全扫描质谱建立其特征ES I-ITM S图谱。结果丹参酮类物质在ES I-M S一级正离子全扫描时,易捕获一个质子形成分子离子,且都易形成二聚体加合N a+离子峰;分子离子在二级质谱中易发生脱水、脱羰基和A环开环裂解。丹参药材中丹参酮类物质的ES I-ITM S图谱重现性与特征性较好,易于解析。结论丹参脂溶性成分ES I-ITM S图可用于丹参药材及复方丹参中丹参酮类成分的快速鉴别。Objective To elucidate the ESI-MS performance of lipophilic tanshinones and to establish a fingerprint of lipophilic tanshinones of Radix Salviae Miltiorrhiza. Methods The lipophilic tanshinones were extracted by ultrasonic wave with 95% ethanol from Radix Salviae Miltiorrhiza and the extracts were analyzed directly in positive ion mode by electrospray ion trap mass spectrometry （ESI-MS）. Results The lipophilic tanshinones were easily to form molecular ions [M＋H]^＋ and dimeric sodium adduct ions [2M＋Na]^＋ in positive ion mode, molecular ions of lipophilic tanshinones were fragmentated through loss ing H2O, CO and A-ring cleavages in ESI-MS^2. The ESLMS fingerprints of lipophilic tanshinone extracts with 14 selected characteristic peaks analyzed with SPSS software were characteristic and stable. Conclusion Lipophilie tanshinones have similar ESI-MS performance; the ESI-MS fingerprint is a useful tool for a rapid and special identification of lipophilic tanshinones in selected traditional Chinese medicine.

关 键 词：丹参药材 脂溶性成分 电喷雾-质谱 指纹图谱 

分 类 号：R282.710[医药卫生—中药学]