大黄对暴发性肝衰竭大鼠肝损伤及肝再生的影响  

Effect of Rhubarb on liver damage and liver regeneration in thioacetamide-induced fulminant hepatic failure in rats

作　　者：朱清静[1] 陈锋[1] 盛国光[1] 

机构地区：[1]湖北省中医院肝病研究所,湖北武汉430061

出　　处：《中国中西医结合消化杂志》2006年第1期5-7,共3页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：湖北省自然科学基金课题资助(2004ABA236)

摘　　要：[目的]探讨大黄对暴发性肝衰竭(FHF)大鼠肝损伤及肝再生的影响。[方法]Wistar大鼠随机分4组:正常对照(对照)组、模型(FHF)组、大黄组、促肝细胞生长素(PHGF)组。FHF、大黄组和PHGF组动物模型采用皮下注射(sc)硫代乙酰胺(TAA)600 mg/kg体重,2次,每次间隔24 h,复制FHF动物模型。大黄组和PHGF组动物除予TAA外,于实验前3天至实验结束分别sc大黄注射液1 ml/100 g和PHGF 1 ml/100 g,对照组和FHF组同时sc 0.85%氯化钠液1 ml/100 g。FHF组、大黄组和PHGF组,于第2次注射TAA后24 h,随机各取8只,腹主动脉取血测肝功能。迅速取肝组织,用10%甲醛液固定,石蜡切片,检测肝细胞有丝分裂指数(MI)和增殖细胞核抗原(PCNA)。[结果]大黄具有降低FHF大鼠丙氨酸氨基转移酶、天冬氨酸转氨酶及总胆红素,并能显著提高MI和PCNA(P<0.05,<0.01)。[结论]大黄具有改善FHF大鼠肝功能和促进肝细胞增殖及再生的作用。[Objective] To study the effect of Rhubarb on liver damage and liver regeneration in thioaeetamide-induced fulminant hepatic failure （FHF） in rats. [Methods] The rats were randomly divided into normal group, model group, Rhubarb group and lyophilized bovine transfer factor control group. Medicines were given 1 ml/100 g respectively 3 days before hypodermically injected with thioacetamide （TAA） once a day. At the same time, the rats were hypodermically injected with TAA 600 mg·k^g-1 twice for two days to induce the model of FHF except those in the normal group which were injected with the 0. 85 % salt solution hypodermically. Forty-eight hours later, the blood was collected to measure ALT, AST, TB and parameters of liver regeneration such as liver mitotic index and proliferation cell nuclear antigen. [-Results] Rhubarb ould obvi- ously decrease ALT, AST, TB and improve liver mitotic index and proliferation cell nuclear antigen of rats with FHF, which has significant difference compared with model group and control group （P〈0.05 or P〈0.01）. [Conclusion]Rhubarb can prevent the development of FHF and improve liver regeneration.

关 键 词：肝衰竭 暴发性 肝再生 增殖细胞核抗原 大黄 

分 类 号：R575[医药卫生—消化系统]