五指山小型猪在GH位点和小卫星位点上与长白猪和枫泾猪的差异  

GENETIC VARIATION AT GH LOCUS AND MINISATELLITE LOCI AMONG WUZHISHAN MINIPIG, LARGE WHITE AND FENGJING PIG BREEDS

作　　者：孟安明[1] 冯书堂[1] 蔡正华[1] 

机构地区：[1]北京农业大学生物学院动物生化教研室,中国农业科学院畜牧研究所

出　　处：《畜牧兽医学报》1996年第5期433-435,共3页ACTA VETERINARIA ET ZOOTECHNICA SINICA

摘　　要：五指山小型猪在ＧＨ位点和小卫星位点上与长白猪和枫泾猪的差异孟安明（北京农业大学生物学院动物生化教研室，北京１０００９４）冯书堂，蔡正华（中国农业科学院畜牧研究所，北京１０００９４）五指山小型猪分布于海南岛五指山区，其成年体重仅２５～３５ｋｇ，而且还具...Abstract The Wuzhishan micropig, with a body weight of 25-35 kg at maturation, was originally distributed in the Wuzhishan Mountains, Hainan Province,China, but it became extinct there a few years ago. This minipig breed has been rescued by the Institute of Animal Science, Chinese Academy of Agricultural Sciences, where a small population(over 100 individuals) has been established by introducing two sows and one boar from the native haunt to Beijing.Since growth hormone(GH) locus is related to the growth of animals, we examined by Southern blotting with a porcine GH cDNA probe the GH locus of the introduced boar and one of the sows and their progeny of this breed as well as individuals of two other breeds (Large White and Fengjing).It was found that, whatever EcoRI, EcoRV or Pst I was used, some of the Wuzhishan minipigs had a strong hybridization band, whereas it was absent in the tested Large White and Fengjing pigs.Whether this difference is related to differentiation in body size of these breeds needs further study, In addition, DNA fingerprinting with human minisatellite 33.15 revealed that similarity coefficient between the introduced boar and sow of the Wuzhishan minipig was 0.632, and that among the progeny population was 0.738±0.047.This suggests that the Wuzhishan minipig might undergo intensive inbreeding and have high tolerance to inbreeding.Similarity coefficient between the Wuzhishan minipig and Large White pigs and between Wuzhishan minipigs and Fengjing pigs was 0.152±0.058 and 0.138±0.059, respectively, indicating that the Wuzhishan minipighas a genetic background dissimilar to other normal pigs.

关 键 词：五指山猪 长白猪 枫泾猪 DNA 指纹 小卫星位点 

分 类 号：S828.2[农业科学—畜牧学]