牙龈卟啉菌蛋白酶rgpA的基因克隆和序列分析  

Cloning and sequencing analysis of gingipain R (rgpA) of porphyromonas gingivalis

作　　者：许静[1] 李昂[1] 苟建重[1] 许援朝[1] 饶国洲[1] 谢红帼 

机构地区：[1]西安交通大学口腔医学院,西安710004

出　　处：《陕西医学杂志》2006年第2期163-165,共3页Shaanxi Medical Journal

基　　金：西安交通大学博士基金BJJ2004108;口腔医院院基金资助;西安市科技攻关计划项目(GG05159)

摘　　要：目的:扩增牙龈卟啉菌蛋白酶rgpA催化结构域(rgpAcd)的基因片断并作鉴定。方法:利用PCR方法,克隆牙龈卟啉菌rgpAcd基因,并将基因片断插入pMD18-TVector,通过限制性酶切和核苷酸序列分析鉴定。结果:克隆基因测序结果与GeneBank数据库中的序列一致。结论:成功克隆了牙龈卟啉菌rgpAcd的基因,为体外表达其活性蛋白奠定了基础。Objective： To clone the catalytic domain of gingipain R （rgpAcd） of Porphyromonas gingivalis （Pg）. Methods： The desired DNA fragment rgpAcd was obtained by PCR and was inserted into pMD18-T Vector. The double-stranded DNA of the positive clone was analyzed hy restriction endonuclease mapping and DNA sequencing. Results： A 1476bp specific fragment was obtained and DNA sequencing showed that the fragment was consistent with those of the published. Conclusion： The rgpAcd of Pg was cloned successfully. The protein of rgpAcd will be obtained for further study.

关 键 词：@牙龈卟啉菌 蛋白酶 克隆 核苷酸序列分析 牙龈卟啉菌 基因克隆 基因片断 催化结构域 PCR方法 限制性酶切 

分 类 号：R512.4[医药卫生—内科学] R780.2[医药卫生—临床医学]