降钙素基因相关肽对正常及应激大鼠胃内胆汁返流的影响  

Effects of Calcitonin Gene-related Peptide on Gastric Bile Reflux in Normal and Stress Rats

作　　者：刘艳[1] 罗和生[1] 李玲[1] 曾涛[1] 

机构地区：[1]武汉大学人民医院消化内科肾病内科,武汉430060

出　　处：《临床消化病杂志》2006年第1期35-37,共3页Chinese Journal of Clinical Gastroenterology

摘　　要：目的探讨降钙素基因相关肽((CGRP)及其拮抗剂(h-CGRP 8-37)与正常和应激情况下大鼠胃内胆汁返流的关系。方法 SD大鼠85只,实验分三部分:第一部分大鼠20只随机分为四组:对照组和CGRP低、中、高剂量组各5只,分别腹腔注射生理盐水(1 ml)和CGRP(10 μg/kg 1ml)、CGRP(30μg/kg 1 ml)和CGRP(1 ml),0．5 h后处死,取胃液测胆汁酸(TBA)浓度。第二部分采用冷束缚应激法,将35只大鼠分为两组,对照组5只,实验组30只,从浸入水中开始取2、4、5、6、8、10 h共6个时段,每个时段各5只,分别检测其胃内胆汁酸的浓度和胃黏膜的溃疡指数(用Guth评分),用放免试剂盒检测幽门区CGRP含量。第三部分大鼠30只随机分为两组:应激组和h-CGRP 8-37组各15只,从浸入水中开始取2、4、6 h共3个时段,每个时段各5只。取胃液测TBA浓度。结果正常大鼠在腹腔注射不同剂量CGRP 0．5 h后胃内胆汁酸浓度明显增高;应激性溃疡大鼠于应激结束后2h(即从应激开始计时6 h)胃内胆汁酸达到峰值,溃疡指数和幽门区CGRP含量分别于应激结束后4 h达到峰值和降到波谷;经幽门局部给予CGRP拮抗剂能显著降低应激性溃疡大鼠胃内胆汁返流程度。结论 CGRP能促进胃内肌汁酸反流,CGRP参与了幽门括约肌舒张功能的调控。Objective To study the effects of caleitonin gene-related peptide（CGRP） and its antagonist of CGRP（ h-CGRP 8-37 ） in normat and stress rats on bile reflux in stomach. Methods The study contained 85 SD rats and three parts. Part one：20 rats were randomly divided into control group and 10μg/kg, 30μg/kg, 50μg,/kg CGRP group. Peritoneal injection 30 rain later,the bile acid in gastric juice was measured. Part two：The stress ulcer model was established by cold,water seaking. 35 rats were divided into 0 h （control group） 2 h ,4 h ,6 h,8 h, 10 h stress group and measured the gastric ulcer index and bile acid in gastric juice. The pyloric local CGRP was measured by a radioimmunoassay detection kit. Part three：30 rats were divided into stress group and h-CGRP 8-37 group. The gastric bile acid were measured after 2 h,4 h,6 h stress. Each hour group have 5 rats. Results The bile acid in CGRP group increased significantly compared with those of control group. The bile acid reached the maximum at 2 hours after finish stress. The ulcer index reached the maximum at 4 hours after finish stress. But the pyloric local CGRP decreased to the minimum at 4 hours after finish stress, And,the bile acid of h-CGRP 8-37 group was less than those of antagonist control group. Conclusion CGRP can promote bile add reflux in the stomach. CGRP affect and regulate the relaxation function of the pyloric sphincter.

关 键 词：降钙素基因相关肽 胆汁返流 应激 

分 类 号：R573[医药卫生—消化系统]