GM_3对HL－60细胞的诱导分化作用及细胞膜流动性的影响  

EFFECT OF ACELL DIFFERENTIATION INDUCER,GANGLIOSIDE GM3,ON THE CELL MEMBRANE FLUIDITY OF A HUMAN PROMYELOCYIC CELL LINE HL-60

作　　者：侯卫红[1] 程育林 姬秀琴[1] 

机构地区：[1]河南医科大学生物化学教研室,河南医科大学第三附属医院,濮阳市安阳地区医院

出　　处：《河南医学研究》1996年第3期244-246,共3页Henan Medical Research

摘　　要：作者将纯化得到的神经节音脂ＧＭ３加到人早幼粒白血病细胞系（ＨＬ－６０）ＤＭＥ／Ｆ１２培养液中，终浓度为５０μＭ，细胞的增殖明显受到抑制，并按单核－巨噬细胞途径定向分化成熟，表现为细胞吞噬功能显著增强，非特异性酯酶活性升高等。细胞的形态也呈现与这种分化相一致的变化。在此基础上，作者探讨了以ＤＰＨ为探针测定ＨＬ－６０细胞膜流动性的实验条件，研究了促分化剂神经节耷脂ＧＭ３、二甲基亚砜（ＤＭＳＯ）和佛波酯（ＴＰＡ）对ＨＬ－６０细胞膜流动性的影响，结果表明：ＧＭ３、ＤＭＳＯ和ＴＰＡ均可降低ＨＬ－６０细胞膜流动性。When human promyelocytic leukemia cell line HL-60 cells were cultured with50μM exogenous GM3 in DME/F12 medium,supplemented with transferrin,insulin,SeO2 and2%calf Serum,cell growth was markedly inhihited.After 6 days of culture,HL-60 cells wereinduced to differentiate along the monocyte-macrophage route.GM3 treatment of HL-60 cellssignificantly increased the percentage of phagocytosing and NSE positive cells.Cells stainedwith wright-Giemsa staining showed morphologically that they were differentiated intomonocyte-macrophage cells.On the basis of this observation,the effects of DMSO,TPA andGM3 on HL-60 cell membrane fluidity were investigated.It was found that DMSO,TPA andGM3 significantly decreased the membrane fluidity of HL-60 cells.

关 键 词：神经节苷脂 HL-60细胞 细胞膜流动性 白血病 

分 类 号：Q546.02[生物学—生物化学] R733.702[医药卫生—肿瘤]