端粒酶在人BEL-7402肝癌细胞中表达的实验研究  

The Study of hTERT Expression in Human BEL-7402 Liver Cancer Cells

作　　者：王铭栋[1] 许德顺[1] 李益生[1] 孙健[1] 

机构地区：[1]锦州医学院附属第一医院检验科,辽宁锦州121001

出　　处：《锦州医学院学报》2005年第6期27-30,共4页Journal of Jinzhou Medical College

摘　　要：目的探讨端粒酶逆转录酶(hTERT)在人BEL-7402肝癌细胞中表达及其临床应用价值。方法采用抗人hTERT单克隆抗体,以免疫组化法定性检测hTERT在肝癌细胞的分布。端粒酶-酶联免疫法(端粒酶-ELISA)半定量检测hTERT蛋白在肝癌细胞的表达。结果hTERT在肝癌细胞中胞核、胞浆都表达,主要以胞核显棕黄色为主。端粒酶-酶联免疫法可半定量检测2×105/μl以上的细胞中hTERT的表达。结论实验结果提示:端粒酶逆转录酶(hTERT)在人BEL-7402肝癌细胞中高表达与肿瘤发生、发展密切相关,可作为一种比端粒酶活性检测更具有癌特异性的检测指标。Objective To investigate the expression of hTERT in human BEL-7402 liver cancer cells and its clinical value. Methods hTERT monoclonal antibody and immunohistochemical techniques were applied to detect the distribution of hTERT in liver cancer. The expression of hTERT protein in liver cancer cells was observed by half quantitative determination of FIT-ELISA method. Results hTERT was expressed in both cytoblast and cytolymph and the brown color of cytoblast was its major expression, hTERT expression in over 2×10^5/μL cells could be detected with FIT-ELISA method. Conclusions The high expression of hTERT in human BEL-7402 liver cancer cells was closely related to the occurrence, development of tumor. It was looked as a type of detection index, which was better than HT active detection in cancer specificity.

关 键 词：端粒酶 端粒酶逆转录酶(hTERT) 肝癌细胞 酶联免疫(ELISA) 免疫组化 

分 类 号：R735.7[医药卫生—肿瘤]