潘托拉唑对胃黏膜损伤保护作用及其机制  

Protective effect of pantoprazole on gastric mucosal lesions in rats andits mechanism

作　　者：乐桥良[1] 张志坚[1] 林克荣[1] 王雯[1] 

机构地区：[1]南京军区福州总医院消化科,福建省福州市350025

出　　处：《世界华人消化杂志》2005年第20期2480-2484,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨潘托拉唑对大鼠胃黏膜损伤保护的作用及其机制.方法:在乙醇诱导大鼠胃黏膜损伤前,预先给予(iv)潘托拉唑(20mg/kg)、L-硝基-精氨酸甲酯(L-NAME,4mg/kg)及L-精氨酸(250mg/kg).采用激光多普勒血流计(LDF)测定胃黏膜血流量(GMBF),采用镉粒还原和比色法测定胃黏膜和血浆NO-2/NO-3含量,并观察了胃黏膜损伤指数(ulcerindex,UI)、溃疡坏死组织和中性粒细胞浸润严重程度的变化.结果:与模型损伤组比,潘托拉唑组大鼠UI明显降低(5.7±2.1vs25.4±2.5,P<0.01),溃疡坏死组织和中性粒细胞浸润程度明显减轻.预先用L-NAME处理后,潘托拉唑保护胃黏膜损伤作用明显减弱;L-NAME抑制作用可被L-精氨酸拮抗.iv潘托拉唑,可增加GMBF、胃黏膜和血浆NO-2/NO-3,L-NAME可逆转这种作用,但对潘托拉唑抑制酸分泌作用无明显影响.结论:潘托拉唑通过一氧化氮介导对大鼠胃黏膜损伤有重要的保护作用,而与潘托拉唑抑制酸分泌作用无关.AIM： To explore the protective effect of pantoprazole on the gastric mucosal lesions in rats and its related mechanism. METHOOS： Before the gastric mucosal lesion was induced by pure alcohol, the rats were intravenously injected with pantoprazole （20 mg/kg）, Nω-nitro- L-arginine methyl ester （L-NAME, 4 mg/kg）, and L-arginine （250 mg/kg）. The gastric mucosal blood flow （GMBF） was detected by laser Doppler flowmetry （LDF）, and the level of NO2^·/NO3^· in the gastric mucosa and serum was measured by cadmium granulation reduction and colorimetric method. The ulcer index （UI）, the severity of tissue necrosis, and neutrophils infiltration were observed. RESULTS： Compared with that in the control group, the UI was significantly decreased （5.7±2.1 vs 25.4± 2.5, P 〈0.01）, and the degrees of tissue necrosis and neutrophils infiltration were markedly lightened. The protective effect of pantoprazole was significantly decreased by L-NAME. The inhibitory effect of L-NAME was antagonized by prior administration of L-arginine. The GMBF and the content of NO2^·/NO3^· in gastric mucosa and serum were increased by the intravenous administration of pantoprazole. This effect was prevented by the pretreatment of L-NAME, but the antisecretion effect of pantoprazole was not affected by L-NAME. CONCLUSION： Pantoprazole can protect gastric mucosa against the lesions in rats by the mediation of nitric oxide （NO）, and the protective effect has no relation with its antisecretory action of gastric acid.

关 键 词：潘托拉唑 胃黏膜损伤 细胞保护 一氧化氮 胃黏膜损伤指数 保护作用 L-硝基-精氨酸甲酯 中性粒细胞浸润 L-NAME 激光多普勒血流计 

分 类 号：R573[医药卫生—消化系统]